Overview

  • Product name
    Anti-active Caspase 7 antibody
  • Description
    Rabbit polyclonal to active Caspase 7
  • Specificity
    This antibody recognizes only the large subunit of cleaved caspase 7. The antibody does not recognize full length caspase 7 or any other caspase.
  • Tested applications
    Suitable for: IHC-Fr, IP, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human active Caspase 7. Synthetic peptide surrounding amino acid 195 of Human Caspase-7.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 7 has been identified as a major contributor to the execution of apoptosis. Caspase 7 is cleaved by many enzymes, including caspase 3, 6, 8, 9 and granzyme B. Active caspase 7 consists of a heterotetramer of two large and two small subunits. As an effector caspase, caspase 7 cleaves many of the same substrates as caspase 3, including poly (ADP-ribose) polymerase (PARP). The active caspase 7 antibody provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 10 - 20 µg/ml.
IP Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa.
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance
    Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • Apoptotic protease Mch3 antibody
    • CASP 7 antibody
    • Caspase 7 subunit p11 antibody
    • Caspase 7 subunit p20 antibody
    • CMH1 antibody
    • ICE LAP3 antibody
    • ICE like apoptotic protease 3 antibody
    see all

Images

  • All lanes : Anti-active Caspase 7 antibody (ab2323) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse hepatocyte cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 20 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Anti-active Caspase 7 antibody (ab2323) at 1 µg/ml
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Jurkat cells induced with 2 mM of Camptothecin for 6 hours

References

This product has been referenced in:
  • Wang-Johanning F  et al. Immunotherapeutic potential of anti-human endogenous retrovirus-k envelope protein antibodies in targeting breast tumors. J Natl Cancer Inst 104:189-210 (2012). WB ; Human . Read more (PubMed: 22247020) »
  • Liu X  et al. Expression and role of the inhibitor of apoptosis protein livin in chemotherapy sensitivity of ovarian carcinoma. Int J Oncol 41:1021-8 (2012). Read more (PubMed: 22766624) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (LIVER)
Specification
LIVER
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Nov 17 2014

I'm sorry to hear you are having a problem with ab2323 I would like to suggest the following modifications to your protocol: block for 1 hour at room temperature in 5% milk, incubate the primary at 1 ug/mL in blocking buffer overnight at 4 degrees,...

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Thank you for your enquiry. According to the datasheet, this antibody has not been tested on formalin-fixed tissue sections (only on frozen sections). Should you decide to go ahead and test this antibody, please let us know how you get on, and in...

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I can tell you that we did not soucre this antibody from cell signalling but our suppliers names are commercially sensitive information. The antibody should be used at 10-20ug/ml in frozen sections and antigen retrieval is not required although it may ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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