Overview

  • Product nameAnti-active Caspase 9 antibody
    See all active Caspase 9 primary antibodies
  • Description
    Rabbit polyclonal to active Caspase 9
  • SpecificityThis antibody recognizes only the cleaved caspase 9 (37 kDa). It does not recognize full-length caspase 9 or any other caspase.
  • Tested applicationsSuitable for: ICC, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Positive control
    • Etoposide treated Jurkat whole cell lysate
  • General notes


    Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase 9 (47 kDa) and Apaf-1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. This affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, 0.5% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesCaspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase 9 (47 kDa) and Apaf-1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. This affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 10 - 20 µg/ml.
IP Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml.

Target

  • Database links
  • Alternative names
    • APAF 3 antibody
    • APAF3 antibody
    • Apoptotic protease activating factor 3 antibody
    • Apoptotic protease MCH 6 antibody
    • CASP9 antibody
    • Caspase 9 antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • Protein phosphatase 1 regulatory subunit 56 antibody
    see all

Anti-active Caspase 9 antibody images

  • All lanes : Anti-active Caspase 9 antibody (ab2324)

    Lane 1 : Jurkat cell lysate, untreated
    Lane 2 : Jurkat cell lysate, treated with etoposide for 6 hours


    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-active Caspase 9 antibody (ab2324)

This product has been referenced in:
  • Staudacher I  et al. HERG K+ channel-dependent apoptosis and cell cycle arrest in human glioblastoma cells. PLoS One 9:e88164 (2014). WB ; Human . Read more (PubMed: 24516604) »
  • Ritch JJ  et al. Multiple phenotypes in Huntington disease mouse neural stem cells. Mol Cell Neurosci 50:70-81 (2012). WB ; Mouse . Read more (PubMed: 22508027) »

See all 5 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Username

Dr. Kirk McManus

Verified customer

Submitted Oct 14 2008

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 26 2014

Thank you for contacting us.

I have had a look at our catalogue and the naming of our antibodies is indeed confusing. We work with different collaborators and they have different ways of naming the antibodies. For example, some of them will ...

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Unfortunately, this product is not covered by our guarantee as it was bought over 6 months ago.

Given that I believe the protocol used should have given satisfactory results and that your customer has spent much time in the lab trying to reso...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vita...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I a...

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Application Flow Cytometry
Sample Human Cell (Jurkat)
Specification Jurkat
Preparation Cell harvesting/tissue preparation method: Spin down, wash in FACS buffer 1x, fix, wash 2x, and stain with primary antibody overnight.
Sample buffer: Buffer composition at analysis was 0.1% sodium azide with FBS in phosphate buffered solution.
Fixation Paraformaldehyde
Permeabilization Yes - Triton X-100 and NP40
Gating Strategy Isolate cell population from plot of SSC-A / FSA-A
Username

Abcam user community

Verified customer

Submitted Sep 15 2011

Thank you for your enquiry. Pro-caspase-9 is an approximately 47 kDa protein that is cleaved at an autoactivation site 315/316 resulting in a 35 kDa fragment and a 12 kDa fragment. ab2324 will recognize the cleaved caspase-9 N-terminal fragment (p3...

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The immunogens are synthetic peptides mapping to the N-terminal of the cleavage site: Asp330 (human) and Asp353 (rat). Unfortunately, the exact sequence of the immunogen remains proprietary.

All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with rat has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"