Overview

  • Product nameAnti-active Caspase 9 antibody
    See all active Caspase 9 primary antibodies
  • Description
    Rabbit polyclonal to active Caspase 9
  • SpecificityThis antibody recognizes only the cleaved caspase 9 (37 kDa/17 kDa). It does not recognize full-length caspase 9 or any other caspase.
  • Tested applicationsSuitable for: ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide from the cleavage site of rat caspase-9

  • General notes


    Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase 9 (47 kDa) and Apaf-1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. This affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesCaspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase 9 (47 kDa) and Apaf-1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. This affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml.

Target

  • Database links
  • Alternative names
    • APAF 3 antibody
    • APAF3 antibody
    • Apoptotic protease activating factor 3 antibody
    • Apoptotic protease MCH 6 antibody
    • CASP9 antibody
    • Caspase 9 antibody
    • Caspase 9 apoptosis related cysteine peptidase antibody
    • ICE LAP6 antibody
    • ICE like apoptotic protease 6 antibody
    • MCH6 antibody
    • PPP1R56 antibody
    • Protein phosphatase 1 regulatory subunit 56 antibody
    see all

Anti-active Caspase 9 antibody images

  • Anti-active Caspase 9 antibody (ab2325) at 0.5 µg/ml + Staurosporine treated PC12 cell lysate

References for Anti-active Caspase 9 antibody (ab2325)

This product has been referenced in:
  • Yang X  et al. Neurodegenerative and Inflammatory Pathway Components Linked to TNF-a/TNFR1 Signaling in the Glaucomatous Human Retina. Invest Ophthalmol Vis Sci 52:8442-54 (2011). WB ; Human . Read more (PubMed: 21917936) »
  • Lasbury ME  et al. Polyamine-mediated apoptosis of alveolar macrophages during Pneumocystis pneumonia. J Biol Chem 282:11009-20 (2007). WB . Read more (PubMed: 17314093) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us. I would say that this sort of experiment is very-very tricky. We unfortunately do not have any protocol available at the moment for manually IHC staining quantification with antibodies. I would suggest ploughing through...

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Thank you for contacting us for technical support. I'm very sorry to hear you are experiencing problems with ab2325. I think the problem you are experiencing is the same as your problem with ab9485. I believe that the problem primarily lies with you...

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Dear Catherine, Thank you for your patience. Regarding ab2325, this antibody does not recognize the human protein. As I mentioned in my last e-mail, you can take a look at ab2324, this antibody reacts with human active Caspase 9.

The immunogens are synthetic peptides mapping to the N-terminal of the cleavage site: Asp330 (human) and Asp353 (rat). Unfortunately, the exact sequence of the immunogen remains proprietary.

Thank you for your patience with my answering your enquiry. I passed your information onto the originator of ab2325 to see if they had any thoughts as to what may be going on with your blots and they provided me with the following information. "The act...

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ab2325 has been tested with PC12 cells for Western blot and rat fetus for IHC.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"