Active E. coli Beta Glucuronidase (GUS) full length protein (ab58415)

Overview

  • Product nameActive E. coli Beta Glucuronidase (GUS) full length protein

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • SpeciesEscherichia coli
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab58415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Activity: >10,000,000 units/g protein. This enzyme does not hydrolyze alpha-glucuronides or beta-glucosides. This preparation is essentially free of sulfatase activity.

  • Applications

    Functional Studies

  • FormLyophilised
  • Additional Notes


    This protein can be used for the enzymatic hydrolysis of glucuronides in urine, prior to analysis by enzyme immunoassay, mass spectrometry, gas chromatography, high performance liquid chromatography, or other means. This enzyme preparation has been shown to be useful for determining the presence of androsterone, 17-hydroxycorticosteroids, and estriol in urine.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: Polyethylene glycol (as stabilizer), 10mM Potassium phosphate, 1mM EDTA, 1mM DTT

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute with 75mM phosphate buffer, pH 6.8, to give a 5mg/ml solution. This solution will be clear to slightly hazy but is still active regardless.

General info

  • Alternative names
    • Beta D glucuronidase
    • Beta G1
    • Beta glucuronidase
    • FLJ39445
    • Glucuronidase beta
    • gurA
    • GUSB
    • MPS 7
    • MPS7
    • UidA
    see all
  • RelevanceReporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.
  • Cellular localizationCytoplasmic

References for Active E. coli Beta Glucuronidase (GUS) full length protein (ab58415)

ab58415 has not yet been referenced specifically in any publications.

Product Wall

We determined the activity from an assay where one unit will liberate 1.0 mg of phenolphthalein from phenolphthalein glucuronide per hour at 37 °C at pH 6.8 (30 minute assay).
The activity is per gram, not per mg. So the activity per mg will be...

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Thank you for your enquiry regarding ab58415. I'm sorry for the delay in responding to your question. This lot contained 17,400,000 units per g of protein, protein was 45% of the total mass of the solid. So for this lot of ab58415, one vial of 25,000 u...

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Our production group prepared a synthetic DNA made from the sequence of the uidA gene from the E. coli K12 strain. The UniProtKB/Swiss-Prot entry for the uidA gene sequence is P05804. Here is the FASTA protein sequence: >sp|P05804|BGLR_ECOLI B...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"