Active E. coli Beta Glucuronidase (GUS) full length protein (ab58415)
- Product nameActive E. coli Beta Glucuronidase (GUS) full length protein
- SourceE. coli
- Amino Acid Sequence
- SpeciesEscherichia coli
- Amino acids0 to 0
Our Abpromise guarantee covers the use of ab58415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Biological activity
Activity: >10,000,000 units/g protein. This enzyme does not hydrolyze alpha-glucuronides or beta-glucosides. This preparation is essentially free of sulfatase activity.
- Additional Notes
This protein can be used for the enzymatic hydrolysis of glucuronides in urine, prior to analysis by enzyme immunoassay, mass spectrometry, gas chromatography, high performance liquid chromatography, or other means. This enzyme preparation has been shown to be useful for determining the presence of androsterone, 17-hydroxycorticosteroids, and estriol in urine.
- Concentration information loading...
Preparation and Storage
- Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: Polyethylene glycol (as stabilizer), 10mM Potassium phosphate, 1mM EDTA, 1mM DTT
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ReconstitutionReconstitute with 75mM phosphate buffer, pH 6.8, to give a 5mg/ml solution. This solution will be clear to slightly hazy but is still active regardless.
- Beta D glucuronidase
- Beta G1
- Beta glucuronidase
- Glucuronidase beta
- MPS 7
- RelevanceReporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Encoded by the E. coli GUS gene (also referred to as uidA), GUS protein is a hydrolase that catalyses the cleavage of a variety of beta-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops.
- Cellular localizationCytoplasmic
References for Active E. coli Beta Glucuronidase (GUS) full length protein (ab58415)
ab58415 has not yet been referenced specifically in any publications.