Active E. coli RecQ DNA Helicase full length protein (ab63813)

Overview

  • Product nameActive E. coli RecQ DNA Helicase full length protein
  • DescriptionActive E. coli RecQ DNA Helicase full length protein

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • SpeciesEscherichia coli
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab63813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Purity> 90 % by SDS-PAGE.
    highly purified by several steps of chromatography
  • FormLiquid
  • Additional Notes


    This protein can be used for: 1) Studies on mechanisms of DNA recombination, replication and repair. 2) Application for genetic engineering; For dissociation of special structure DNA.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 50mM Potassium chloride, 20mM Tris HCl, 1mM EDTA, 1mM DTT, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General info

  • Alternative names
    • ATP dependent DNA helicase
    • ATP dependent DNA helicase recQ
    • DNA helicase
    • ECK3816
    • JW5855
    • RecQ
    see all
  • RelevanceRecQ is a DNA helicase, involved in the recF recombination pathway; its gene expression is under the regulation of the SOS system.
  • Cellular localizationCytoplasmic, Bacterial nucleoid, Replisome

Active E. coli RecQ DNA Helicase full length protein images

  • SDS Page analysis of RecQ DNA Helicase (ab63813)

References for Active E. coli RecQ DNA Helicase full length protein (ab63813)

This product has been referenced in:
  • Suhasini AN  et al. DNA repair and replication fork helicases are differentially affected by alkyl phosphotriester lesion. J Biol Chem 287:19188-98 (2012). Read more (PubMed: 22500020) »
  • Hishida T  et al. Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev 18:1886-97 (2004). Read more (PubMed: 15289460) »

See all 2 Publications for this product

Product Wall



I have contacted the lab regarding your enquiry. They have confirmed that RecQ helicase activity will depend on structures of DNA duplex.

The following paper describes details of assay conditions with various forms of DNA substrates ...

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Thank you for contacting us.

This protein corresponds to the Swissprot ID P15043. http://www.uniprot.org/uniprot/P15043

The molecular weight is 68kDa for unprocessed protein. The observed molecular weight as seen in image is 64 kda...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"