Active hepatitis c virus Hepatitis C Virus NS3 protein fragment (ab91395)

Overview

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • SpeciesHepatitis C Virus
    • Sequenceab91395 contains HCV NS3 immunodominant region (1192-1459aa).
    • Amino acids1192 to 1459

Specifications

Our Abpromise guarantee covers the use of ab91395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityab91395 is immunoreactive with sera of HCV-infected individuals.
  • Applications

    SDS-PAGE

    Western blot

    ELISA

  • Purity> 95 % by SDS-PAGE.
    ab91395 is purified by proprietary chromatographic techniques. Purity: >95% by SDS-PAGE.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 0.2% Triton-X-100, 1.5M Urea, 25mM Tris HCl, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General info

  • Alternative names
    • Hepatitis C Virus NS3
    • NS3
    • NS3P
    • p70
    • Serine protease/NTPase/helicase
    see all
  • RelevanceHCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
  • Cellular localizationEndoplasmic reticulum membrane

References for Active hepatitis c virus Hepatitis C Virus NS3 protein fragment (ab91395)

ab91395 has not yet been referenced specifically in any publications.

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I can confirm that both these recombinant HCV NS3 proteins (ab84346 and ab91395) contain an additionalN terminal Methionine.

I hope this will be helpful to you. If you have any fur...

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