Overview

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • SpeciesHuman
    • Molecular weight27 kDa

Specifications

Our Abpromise guarantee covers the use of ab9794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Other

    Cellular activation

    SDS-PAGE

    Western blot

  • FormLyophilised
  • Additional NotesThe ED50 was determined by its ability to stimulate chick E8 neurite outgrowth. The expected ED50 for this effect is 0.1-1.0 ng/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.

General info

  • Alternative names
    • Abrineurin
    • BDNF
    • BDNF
    • BDNF_HUMAN
    • Brain Derived Neurotrophic Factor
    • Brain-derived neurotrophic factor
    • MGC34632
    • MGC34632
    • Neurotrophin
    see all
  • FunctionDuring development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
  • Tissue specificityBrain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
  • Involvement in diseaseCongenital central hypoventilation syndrome
  • Sequence similaritiesBelongs to the NGF-beta family.
  • Post-translational
    modifications
    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P23560 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

References for Active human BDNF full length protein (ab9794)

This product has been referenced in:
  • Wong J & Garner B Evidence that truncated TrkB isoform, TrkB-Shc can regulate phosphorylated TrkB protein levels. Biochem Biophys Res Commun 420:331-5 (2012). Read more (PubMed: 22425982) »
  • Samartgis JR  et al. Brain-derived neurotrophic factor facilitates memory consolidation and reconsolidation of a weak training stimulus in the day-old chick. Neurosci Lett 516:119-23 (2012). Functional Studies . Read more (PubMed: 22484543) »

See all 3 Publications for this product

Product Wall


I heard back from both labs that produce the BDNF and pro-BDNF proteins, and both said that the reconstitution instructions should be followed directly.
BDNF ab9794: reconstitute in water
pro BDNF ab138787: reconstitute in PBS

Thank you for contacting us.
The lab let me know the following:
The protein is lyophilized with no additives, which means that it is HPLC purified. Therefore, the only buffer that would be present at lyophilization would be water and 0.1% TFA ...

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Vielen Dank für Ihre Antwort und für diese weiteren Informationen.

Vorweg: Den Rabattcode können Sie ab sofort nutzen. Da der vorige unseren Unterlagen zufolge gerade abgelaufen ist, habe ich Ihnen nochmal einen neuen ausgestel...

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Vielen Dank für Ihre Anfrage.

Es tut mir leid zu hören, dass Sie Probleme mit unserem BDNF-Antikörper und Proteinhaben.

Bezüglich des Gutscheincodes kann ich Ihnen mitteilen, dass ich die Ausnahme machen konnte, ...

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Thank you for your phone call. I am sending you the testing discount codes for the 2 proteins for trying them in a blocking experiment - see below.

Please send me your WB data so that I cantake a look at your results.
DISCOUNT CODE: xxx ...

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Thank you for contacting us. The active form of BDNF is a 27.0 kDa, dimer formed by two identical 119 amino acid subunits that is held together by strong hydrophobic interactions: HSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKE...

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DISCOUNT CODE:xxxx Expiration date: dd.mm.2012 I am very pleased to hear you would like to use ab9794 and send us an image of your results. This code will give you 1 free protein before the expiration date. To redeem this offer, please submit an...

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Thank you very much for your call. Firstly, I would like to mention that we also have a PC12 cell lysate available that could be used as positive control for ab108383, as stated on the datasheet. I just point this out as a lysate may be of use as a ...

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Thank you for your enquiry and patience. Our source for this protein has told me that yes, the protein can be reconstituted to a concentration of 1 mg/ml with water. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"