Active human c Abl protein fragment (ab69810)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected sf9 cells
  • Amino Acid Sequence
    • AccessionP00519
    • SpeciesHuman
    • Molecular weight135 kDa
    • Amino acids27 to 1130
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab69810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific activity: 871 nmol/min/mg

  • Applications

    Functional Studies

    SDS-PAGE

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General info

  • Alternative names
    • Abelson Murine Leukemia Viral Oncogene Homolog 1
    • Abelson murine leukemia viral v abl oncogene homolog 1
    • Abelson tyrosine protein kinase 1
    • ABL
    • Abl 1
    • Abl protein
    • Abl1
    • ABL1_HUMAN
    • bcr/abl
    • Bcr/c abl oncogene protein
    • c abl oncogene 1 non receptor tyrosine kinase
    • c abl oncogene 1 receptor tyrosine kinase
    • JTK 7
    • JTK7
    • p150
    • Proto oncogene tyrosine protein kinase ABL1
    • Proto-oncogene c-Abl
    • Transformation gene oncogene ABL
    • Tyrosine-protein kinase ABL1
    • v abl
    • v abl Abelson murine leukemia viral oncogene homolog 1
    see all
  • FunctionProtein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.
  • Tissue specificityWidely expressed.
  • Involvement in diseaseNote=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
    Isoform IB is myristoylated on Gly-2.
  • Cellular localizationCytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear.
  • Information by UniProt

Active human c Abl protein fragment images

  • Kinase activity assay of ab69810. The specific activity of c-Abl was determined to be 871 nmol/min/mg.
  • SDS-PAGE of ab69810. Molecular weight 135kDa.

References for Active human c Abl protein fragment (ab69810)

ab69810 has not yet been referenced specifically in any publications.

Product Wall

This protein corresponds to amino acids 27-1130 of the human protein. It also includes 9 amino acids of a His tag fused to the N-terminus.

This protein should therefore been seen at ˜135 kDa on a blot. It is not a mutated version of the prote...

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Yes, HEPES pH 7.2 can be substituted for MOPS in the kinase assay buffer, at the same concentration, 25mM.

Please find attached the functional assay (kinase activity) for ab69810, which includes buffer recommendations.

Please let me know if you have any questions.

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I apologize for any confusion. I have checked with our products department who have confirmed that this was a duplicate in our catalog, ab84546 and ab69810 are both the same.

We have therefore removed a...

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