Active human Calpain 1 full length protein (ab91019)

Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab91019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityActivity: Greater than or equal to 1,000 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one micromole of Suc-LLVY-AMC per minute at 25°C.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity> 95 % by SDS-PAGE.

  • FormLiquid
  • Additional NotesPrepared from whole blood shown to be non-reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 20mM Imidazole
    Constituents: 30% Glycerol, 5mM Beta mercaptoethanol, 1mM EDTA, 1mM EGTA, pH 6.8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General info

  • Alternative names
    • Ca2 activated neutral protease
    • Calcium activated neutral proteinase
    • Calcium activated neutral proteinase small subunit
    • Calcium dependent protease small subunit
    • Calcium dependent protease small subunit 1
    • Calcium-activated neutral proteinase 1
    • Calpain 1
    • Calpain 1 large subunit
    • Calpain mu type
    • Calpain mu-type
    • Calpain regulatory subunit
    • Calpain small subunit 1
    • Calpain-1 catalytic subunit
    • Calpain-1 large subunit
    • CAN1_HUMAN
    • CANP
    • CANP 1
    • CANP small subunit
    • CANPL 1
    • CAPN 1
    • CAPN1
    • Cell proliferation-inducing gene 30 protein
    • Micromolar Calpain
    • Micromolar-calpain
    • Mu Calpain
    • muCANP
    • muCL
    see all
  • FunctionCalcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 4 EF-hand domains.
  • Cellular localizationCytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
  • Target information above from: UniProt accession P07384 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Active human Calpain 1 full length protein images

  • SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
    1. 5 µg Calpain 1 (reduced /heated)
    2. 10 µg Calpain 1 (reduced/ heated)
    3. Molecular weight markers
    4. 5 µg Calpain 1 (non-reduced/no heat)
    5. 10 µg Calpain 1 (non-reduced/no heat)
  • Anti-Calpain 1 antibody (ab2543) at 1 µg/ml + Active human Calpain 1 full length protein (ab91019) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Active human Calpain 1 full length protein (ab91019)

ab91019 has not yet been referenced specifically in any publications.

Product Wall

The specific activity for GR23778 is 2126 Units/mg.

Hello. No, this is not exactly the same, the protein ab91019 comes from another source. They are very similar though. Please let me know if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"