Active human Caspase 8 full length protein (ab54117)
- Product nameActive human Caspase 8 full length proteinSee all Caspase-8 proteins and peptides ...
- SourceE. coli
- Amino Acid Sequence
- Amino acids0 to 0
Our Abpromise guarantee covers the use of ab54117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Biological activitySpecific Activity: 5000 units/mg. One unit of the recombinant Caspase 8 is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
- Purity> 95
% by SDS-PAGE.
- Concentration information loading...
Preparation and Storage
- Stability and Storage
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 15% Glycerol, PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ReconstitutionReconstitute to 1 unit per µl in PBS containing 15% glycerol.
- Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein
- Apoptosis related cysteine peptidase
- Apoptotic cysteine protease
- Apoptotic protease Mch 5
- Apoptotic protease Mch-5
- Apoptotic protease Mch5
- CAP 4
- CASP 8
- Caspase 8
- Caspase 8 apoptosis related cysteine peptidase
- Caspase-8 subunit p10
- CED 3
- FADD homologous ICE/CED 3 like protease
- FADD Homologous ICE/CED3 Like Protease
- FADD Like ICE
- FADD-homologous ICE/CED-3-like protease
- FADD-like ICE
- ICE like apoptotic protease 5
- ICE-like apoptotic protease 5
- MACH alpha 1/2/3 protein
- MACH beta 1/2/3/4 protein
- MCH 5
- MORT1 associated CED 3 homolog
- MORT1 associated CED3 homolog
- MORT1-associated CED-3 homolog
- FunctionMost upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
- Tissue specificityIsoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
- Involvement in diseaseDefects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.
- Sequence similaritiesBelongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.
- DomainIsoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
modificationsGeneration of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Cellular localizationCytoplasm.
References for Active human Caspase 8 full length protein (ab54117)
ab54117 has not yet been referenced specifically in any publications.