Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab90387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityActivity: Greater than 200 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one µmole of Z-Arg-Arg-beta-naphthylamide per minute at 40°C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.
  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Purity> 95 % by SDS-PAGE.

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Sodium acetate, 1mM EDTA, pH 5.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General info

  • Alternative names
    • Amyloid precursor protein secretase
    • APP secretase
    • APPS
    • CATB_HUMAN
    • Cathepsin B heavy chain
    • Cathepsin B1
    • CathepsinB
    • CPSB
    • CTSB
    • cysteine protease
    • OTTHUMP00000116009
    • OTTHUMP00000229510
    • OTTHUMP00000229511
    • OTTHUMP00000229512
    • OTTHUMP00000229514
    • OTTHUMP00000229515
    • OTTHUMP00000229516
    • Preprocathepsin B
    see all
  • FunctionThiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
  • Sequence similaritiesBelongs to the peptidase C1 family.
  • Cellular localizationLysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P07858 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Active human Cathepsin B full length protein images

  • Anti-Cathepsin B antibody [CA10] (ab58802) at 0.25 µg/ml + Active human Cathepsin B full length protein (ab90387) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Active human Cathepsin B full length protein (ab90387)

This product has been referenced in:
  • Yoon JY  et al. Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B. Blood Cancer J 3:e153 (2013). Read more (PubMed: 24141622) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"