Active human Eph receptor B2 protein fragment (ab42607)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceF ERADSEYTDK LQHYTSGHMT PGMKIYIDPF TYEDPNEAVR EFAKEIDISC VKIEQVIGAG EFGEVCSGHL KLPGKREIFV AIKTLKSGYT EKQRRDFLSE ASIMGQFDHP NVIHLEGVVT KSTPVMIITE FMENGSLDSF LRQNDGQFTV IQLVGMLRGI AAGMKYLADM NYVHRDLAAR NILVNSNLVC KVSDFGLSRF LEDDTSDPTY TSALGGKIPI RWTAPEAIQY RKFTSASDVW SYGIVMWEVM SYGERPYWDM TNQDVINAIE QDYRLPPPMD CPSALHQLML DCWQKDRNHR PKFGQIVNTL DKMIRNPNSL KAMAPLSSGI NLPLLDRTIP DYTSFNTVDE WLEAIKMGQY KESFANAGFT SFDVVSQMMM EDILRVGVTL AGHQKKILNS IQVMRAQMNQ IQSVEGQPLA RRPRATGRTK RCQPRDVTKK TCNSNDGKKK GMGKKKTDPG RGREIQGIFF KEDSHKESND CSCGG
    • Amino acids569 to 1055

Specifications

Our Abpromise guarantee covers the use of ab42607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityActivity: 150 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of tyrosine substrate per minute at pH 7.4 and 30C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 4 ug /ml recombinant EPHB2.
  • Applications

    Inhibition Assay

  • FormLiquid
  • Additional NotesExpressed in a Baculovirus infected Sf9 expression system. Expected molecular weight of 52 kDa.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General info

  • Alternative names
    • cek5
    • Developmentally regulated EPH related tyrosine kinase
    • DRT
    • EK5
    • ELK related protein tyrosine kinase
    • Eph receptor B2
    • EPH tyrosine kinase 3
    • EPH-like kinase 5
    • EPHB2
    • EPHB2_HUMAN
    • Ephrin type B receptor 2
    • Ephrin type-B receptor 2
    • EPHT 3
    • ERK
    • ETECK
    • hEK5
    • Nuk
    • Prkm 5
    • Receptor protein tyrosine kinase HEK 5
    • Renal carcinoma antigen NY-REN-47
    • Sek 3
    • Tyro 5
    • Tyrosine protein kinase receptor CEK 5
    • Tyrosine protein kinase receptor EPH 3
    • Tyrosine protein kinase receptor QEK 5
    • Tyrosine-protein kinase receptor EPH-3
    • Tyrosine-protein kinase TYRO5
    see all
  • FunctionReceptor for members of the ephrin-B family. Phosphorylates ARHGEF15, leading to its ubiquitination and degradation by the proteasome which promotes EFNB1-dependent synapse formation. Can function in aspects of retinal ganglion cell axon guidance to the optic disk even when lacking its tyrosine kinase domain. Acts as a tumor suppressor.
  • Tissue specificityBrain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.
  • Involvement in diseaseDefects in EPHB2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Note=EPHB2 mutations have been found in a prostate cancer cell line derived from a brain metastasis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession P29323 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

References for Active human Eph receptor B2 protein fragment (ab42607)

ab42607 has not yet been referenced specifically in any publications.

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