The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The ED50 of ab88394 is typically 0.05-0.1 ng/ml as measured in a cell proliferation assay using human umbilical endothelial cells (HUVECs).
% by SDS-PAGE.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Basic fibroblast growth factor
Basic fibroblast growth factor bFGF
Fibroblast growth factor 2
Fibroblast growth factor 2 (basic)
Fibroblast growth factor, basic
Heparin binding growth factor 2 precursor
Heparin-binding growth factor 2
FunctionPlays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
Tissue specificityExpressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
Sequence similaritiesBelongs to the heparin-binding growth factors family.
Post-translational modificationsPhosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
Cellular localizationSecreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
1D SDS-PAGE of ab88394 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab88394
Lane 2: ab88394 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab88394 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
Additional bands in lane 2 and lane 3 are glycosidase enzymes. The results suggest that ab88394 is not glycosylated.
References for Active human FGF basic full length protein (ab88394)
has not yet been referenced specifically in any publications.
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