Active human G-CSF full length protein (ab73371)

Overview

Description

  • NatureRecombinant
  • SourceCHO
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Thr-Pro-Leu-Gly-Pro.
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab73371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activitySpecific Activity of 1.27 x 108 IU/mg.
  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Purity> 95 % by SDS-PAGE.
    ab73371 was purified by proprietary chromatographic techniques. Purity is greater than 98.0% as determined by RP-HPLC and SDS-PAGE.
  • FormLyophilised
  • Additional NotesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10mM Hydrochloric Acid, Mannitol, Tween 20, L-arginine, phenylalanine and methionine. pH 6.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute in sterile 18MOhm/cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

General info

  • Alternative names
    • C17orf33
    • Colony stimulating factor 3 (granulocyte)
    • CSF 3
    • CSF beta
    • CSF3
    • CSF3_HUMAN
    • CSF3OS
    • Csfg
    • Filgrastim
    • G-CSF
    • GCSA
    • GCSF
    • Granulocyte colony stimulating factor
    • Granulocyte colony-stimulating factor
    • Lenograstim
    • Macrophage granulocyte inducer 2
    • MGC45931
    • MGI 2
    • Pluripoietin
    see all
  • FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    modifications
    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P09919 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Active human G-CSF full length protein images

  • Anti-G-CSF antibody (ab9691) at 1 µg/ml + Active human G-CSF full length protein (ab73371) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes

References for Active human G-CSF full length protein (ab73371)

ab73371 has not yet been referenced specifically in any publications.

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