Active human GCKR full length protein (ab123840)

Overview

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • AccessionP35557
    • SpeciesHuman
    • Molecular weight53 kDa including tags
    • Amino acids0 to 0
    • TagsDDDDK tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab123840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity303 pmol/min/µg. One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30°C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 µg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30°C for 30 min.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity> 80 % by SDS-PAGE.

  • FormLiquid
  • Additional Notes 303 pmol/min/µg. One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30°C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 µg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30°C for 30 min.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.40
    Constituents: 1.06% Potassium phosphate, 0.008% DTT, 0.015% EDTA, 2.5% Glycerol, 0.29% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General info

  • Alternative names
    • FGQTL5
    • GCK
    • GCKR
    • GCKR_HUMAN
    • GKRP
    • GLRE
    • Glucokinase
    • glucokinase (hexokinase 4) regulator
    • Glucokinase regulator
    • Glucokinase regulatory protein
    • Hexokinase type IV
    • Hexokinase-4
    • Hexokinase-D
    • HK IV
    • HK4
    • OTTHUMP00000158533
    see all
  • FunctionInhibits glucokinase by forming an inactive complex with this enzyme.
  • Tissue specificityFound in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.
  • Sequence similaritiesBelongs to the GCKR family.
    Contains 2 SIS domains.
  • Target information above from: UniProt accession Q14397 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Active human GCKR full length protein images

  • SDS-PAGE showing ab123840 (10µg).

References for Active human GCKR full length protein (ab123840)

ab123840 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"