Active human GDF8 / Myostatin protein fragment (ab50097)

Overview

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • AccessionO14793
    • SpeciesHuman
    • SequenceDFGLDCDEHS TESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGECEFV FLQKYPHTHL VHQANPRGSA GPCCTPTKMS PINMLYFNGK EQIIYGKIPA MVVDRCGCS
    • Molecular weight25 kDa
    • Amino acids267 to 375

Specifications

Our Abpromise guarantee covers the use of ab50097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected ED50 for this effect is 17.0-25.0 ng/ml.

  • Applications

    Inhibition Assay

    SDS-PAGE

    Functional Studies

  • Purity> 95 % by SDS-PAGE.
    Greater than 98% by SDS-PAGE and HPLC analyses.
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.

General info

  • Alternative names
    • GDF 8
    • GDF-8
    • GDF8
    • GDF8_HUMAN
    • Growth differentiation factor 8
    • Growth/Differentiation Factor 8
    • MSLHP
    • MSTN
    • myostatin
    • OTTHUMP00000163498
    see all
  • FunctionActs specifically as a negative regulator of skeletal muscle growth.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Active human GDF8 / Myostatin protein fragment (ab50097)

ab50097 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

These two proteins are the same, except for the reconstitution instructions (water versus 20 mM Hcl). Both should work equally well in inhiibiting MPC-11 cell growth.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"