Active human Lactate Dehydrogenase Isoenzyme V full length protein (ab77945)

Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab77945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Specific Activity: 150 Units/mg protein. One unit will catalyze the transformation of one micromole of Lactate to pyruvate at 37°C and pH 8.55

  • Applications

    Western blot

    Functional Studies

  • Purity= 100 % Ammonium Sulphate Precipitation.
    ab77945 is 100% pure as determined by electrophoresis. Contaminants: ALP: 0.002%, GOT: 0.20%, GGT: <0.001%, GPT: 0.01%.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Store at +4°C. Do not freeze.

    Preservative: None
    Constituents: 50mM Tris-Chloride, 1mM EDTA, 1mM DTE, pH 8.3

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General info

  • Alternative names
    • Cell proliferation-inducing gene 19 protein
    • L lactate dehydrogenase A chain
    • Lactate dehydrogenase 5
    • Lactate dehydrogenase A
    • Lactate dehydrogenase isoenzyme 5
    • Lactate dehydrogenase V
    • LDH 5
    • LDH A
    • LDH M
    • LDH muscle subunit
    • LDH1
    • LDH5
    • LDHA
    • NY REN 59 antigen
    • PIG 19
    • PIG19
    • Proliferation inducing gene 19 protein
    • Renal carcinoma antigen NY REN 59
    • Renal carcinoma antigen NY-REN-59
    see all
  • RelevanceLactate dehydrogenase 5 (LDH5) is one of five LDH isoenzymes and important for promoting anaerobic glycolysis. LDH5 is transcriptionally regulated by the hypoxia inducible factors (HIF) 1a and 2a.
  • Cellular localizationCytoplasmic

Active human Lactate Dehydrogenase Isoenzyme V full length protein images

  • Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab85472) at 1 µg/ml + Active human Lactate Dehydrogenase Isoenzyme V full length protein (ab77945) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 12 minutes

References for Active human Lactate Dehydrogenase Isoenzyme V full length protein (ab77945)

ab77945 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your phone call.
As we discussed I am sending you the 3 discount codes for the 2 antibodies as well as the one protein, all of which have not yet been tested in sandwich ELISA.
DISCOUNT CODE: xxx for ab85472
DISCOUNT CODE: ...

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Thank you for your enquiry. Sorry for the confusing information appearing on the datasheet for ab77945. The actual concentration is 7.5mg/ml and the product activity is 1140 units/ml. I hope this is helpful. Please contact me again if you have any furt...

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Here is the information specific for ab77945 Lot GR54583-2:    Activity:  1,140 Unit/ml @ 37°C Dimension Protein:  7.5 mg Protein/ml (Coomassie) Specific Activity: 150 Units/mg protein One unit will catalyze the oxidation of...

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Thanks for your inquiry. The new Lot GR54583-2 is at a higher concentration and therefore the 100 Unit package size will fit in a smaller vial compared to the previous lot. New Lot : concentration 1140 Units/mL = 88uL per 100 Units ...

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Thanks for your call today and for letting us know about the problem with ab77945. As we discussed, I am sending a new vial which should arrive tomorrow. Please keep me updated about how this protein works in your assay. Also, if you have any d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"