Active human PKC epsilon full length protein (ab60847)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab60847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 0.1M PMSF, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General info

  • Alternative names
    • KPCE_HUMAN
    • MGC125656
    • MGC125657
    • nPKC epsilon
    • nPKC-epsilon
    • PKCE
    • Pkcea
    • PRKCE
    • Protein kinase C epsilon
    • Protein kinase C epsilon type
    see all
  • FunctionThis is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Post-translational
    modifications
    Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.
  • Information by UniProt

Active human PKC epsilon full length protein images

  • Sample Kinase Activity Plot.
  • ab60847 on SDS-PAGE, MW ~110 kDa.

References for Active human PKC epsilon full length protein (ab60847)

ab60847 has not yet been referenced specifically in any publications.

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