Active human PTP epsilon protein fragment (ab128555)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected sf9 cells
  • Amino Acid Sequence
    • AccessionP23469
    • SpeciesHuman
    • Molecular weight91 kDa including tags
    • Amino acids75 to 700

Specifications

Our Abpromise guarantee covers the use of ab128555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe specific activity of ab128555 was determined to be 8,600 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity>95% by SDS-PAGE .

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.02% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General info

  • Alternative names
    • DKFZp313F1310
    • FLJ57799
    • FLJ58245
    • HPTPE
    • Protein tyrosine phosphatase epsilon
    • Protein tyrosine phosphatase receptor type E
    • Protein tyrosine phosphatase receptor type epsilon
    • Protein tyrosine phosphatase receptor type epsilon polypeptide
    • Protein-tyrosine phosphatase epsilon
    • PTPE
    • Ptpre
    • PTPRE_HUMAN
    • R PTP Epsilon
    • R-PTP-epsilon
    • Receptor-type tyrosine-protein phosphatase epsilon
    see all
  • FunctionIsoform 1 plays a critical role in signaling transduction pathways and phosphoprotein network topology in red blood cells. May play a role in osteoclast formation and function.
    Isoform 2 acts as a negative regulator of insulin receptor (IR) signaling in skeletal muscle. Regulates insulin-induced tyrosine phosphorylation of insulin receptor (IR) and insulin receptor substrate 1 (IRS-1), phosphorylation of protein kinase B and glycogen synthase kinase-3 and insulin induced stimulation of glucose uptake.
    Isoform 1 and isoform 2 act as a negative regulator of FceRI-mediated signal transduction leading to cytokine production and degranulation, most likely by acting at the level of SYK to affect downstream events such as phosphorylation of SLP76 and LAT and mobilization of Ca(2+).
  • Tissue specificityExpressed in giant cell tumor (osteoclastoma rich in multinucleated osteoclastic cells).
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 4 subfamily.
    Contains 2 tyrosine-protein phosphatase domains.
  • DomainThe tyrosine-protein phosphatase 2 domain (D2) mediates dimerization. The extreme N- and C- termini of the D2 domain act to inhibit dimerization and removal of these sequences increases dimerization and inhibits enzyme activity.
  • Post-translational
    modifications
    A catalytically active cytoplasmic form (p65) is produced by proteolytic cleavage of either isoform 1, isoform 2 or isoform 3.
    Isoform 1 and isoform 2 are phosphorylated on tyrosine residues by tyrosine kinase Neu.
    Isoform 1 is glycosylated.
  • Cellular localizationCytoplasm; Cell membrane and Cytoplasm. Predominantly cytoplasmic. A small fraction is also associated with nucleus and membrane. Insulin induces translocation to the membrane.
  • Target information above from: UniProt accession P23469 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Active human PTP epsilon protein fragment images

  • SDS Page analysis of ab128555
  • The specific activity of ab128555 was determined to be 8,600 nmol/min/mg.

References for Active human PTP epsilon protein fragment (ab128555)

ab128555 has not yet been referenced specifically in any publications.

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