Active human SYVN1 protein fragment (ab125740)

Overview

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • AccessionQ86TM6
    • SpeciesHuman
    • Molecular weight39 kDa
    • Amino acids246 to 617

Specifications

Our Abpromise guarantee covers the use of ab125740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThis N terminal deletion mutant is active as an E3 ligase containing the RING and substrate recognition domains. Typical enzyme concentration of 20-1000nM is used for in vitro conjugation depending on assay conditions.
  • Applications

    Functional Studies

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

    Constituents: 89% PBS, 10% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General info

  • Alternative names
    • 1200010C09Rik
    • DER3
    • E3 ubiquitin protein ligase synoviolin
    • E3 ubiquitin-protein ligase synoviolin
    • HMG coA reductase degradation 1 homolog
    • HRD1
    • KIAA1810
    • MGC40372
    • OTTHUMP00000230429
    • OTTHUMP00000230430
    • OTTHUMP00000230431
    • OTTHUMP00000230432
    • Synovial apoptosis inhibitor 1
    • Synovial apoptosis inhibitor 1, synoviolin
    • SYNOVIOLIN
    • Synoviolin 1 isoform b
    • SYVN1
    • SYVN1_HUMAN
    see all
  • FunctionActs as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis.
  • Tissue specificityUbiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level).
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the HRD1 family.
    Contains 1 RING-type zinc finger.
  • DomainThe RING-type zinc finger is required for E3 ligase activity.
  • Post-translational
    modifications
    Not N-glycosylated.
    Auto-ubiquitinated.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Target information above from: UniProt accession Q86TM6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

References for Active human SYVN1 protein fragment (ab125740)

ab125740 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab125740.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"