Active human TGF beta 1 full length protein (ab50038)

Overview

Description

  • NatureRecombinant
  • SourceCHO
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab50038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityBiological Activity : The ED50 was determined by TGF beta 1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is < 0.05 ng/ml, corresponding to a specific activity of >2 x 107 units/mg
  • Applications

    SDS-PAGE

    Functional Studies

  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

General info

  • Alternative names
    • Camurati Engelmann disease
    • CED
    • Diaphyseal dysplasia 1 progressive
    • DPD1
    • LAP
    • Latency-associated peptide
    • TGF beta
    • TGF beta 1
    • TGF beta 1 protein
    • TGF-beta 1 protein
    • TGF-beta-1
    • TGF-beta-5
    • TGF-beta1
    • TGFB
    • TGFB 1
    • Tgfb-1
    • TGFB1
    • TGFB1_HUMAN
    • TGFbeta
    • TGFbeta1
    • Transforming Growth Factor b1
    • Transforming Growth Factor beta 1
    • Transforming growth factor beta 1a
    • transforming growth factor beta-1
    • transforming growth factor, beta 1
    see all
  • FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Post-translational
    modifications
    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P01137 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

References for Active human TGF beta 1 full length protein (ab50038)

ab50038 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry.

I have reviewed with our sources the TGF beta 1 proteins that we have on our catalog. It seems the most suitable would be ab50036 TGF beta 1 protein (Active).

http://www.abcam.com/index.html?datasheet=5...

Read More

Thank you for contacting us.

I assume you would like to use the protein for functional studies? From the looks of it, all three of them seem to be suitable for Functional Studies, but withinAb50036the endotoxin level has been determined as w...

Read More

The TGF-beta 1 is secreted from the CHO cells, the media is then collected and the protein is isolated and purified. Please do not hesitate to contact me should you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"