Recombinant human TGF beta 2 protein (Active) (ab84070)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, Functional Studies, SDS-PAGE, WB
Achieve higher consistency and quality standards with a premium grade bioactive protein
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
Description
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Product name
Recombinant human TGF beta 2 protein (Active)
See all TGF beta 2 proteins and peptides -
Biological activity
Determined by its ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells.
The ED50 was found to be = 0.2 ng/ml, corresponding to a specific activity of = 5 x 106 units/mg.
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Purity
> 98 % SDS-PAGE.
> 98% HPLC analyses. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
ALDAAYCFRN VQDNCCLRPL YIDFKRDLGW KWIHEPKGYN ANFCAGACPY LWSSDTQHSR VLSLYNTINP EASASPCCVS QDLEPLTILY YIGKTPKIEQ LSNMIVKSCK CS -
Predicted molecular weight
25 kDa -
Amino acids
303 to 414
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Specifications
Our Abpromise guarantee covers the use of ab84070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
HPLC
Functional Studies
SDS-PAGE
Western blot
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Stable for 12 months at -20°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute in water to 0.1-1.0 mg/ml. Store at 2°C to 8°C for up to 1 week or prepare for extended storage. After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20°C to -80°C.
General Info
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Alternative names
- BSC-1 cell growth inhibitor
- BSC1 cell growth inhibitor
- Cetermin
see all -
Function
TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. -
Involvement in disease
A chromosomal aberration involving TGFB2 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with HDAC9.
Loeys-Dietz syndrome 4
Defects in TGFB2 may be a cause of non-syndromic aortic disease (NSAD). NSAD is a frequently asymptomatic but potentially lethal disease characterized by thoracic aortic aneurysms and dissections without additional syndromic features. -
Sequence similarities
Belongs to the TGF-beta family. -
Post-translational
modificationsThe precursor is cleaved into mature TGF-beta-2 and LAP, which remains non-covalently linked to mature TGF-beta-2 rendering it inactive. -
Cellular localization
Secreted. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (8)
ab84070 has been referenced in 8 publications.
- Li Q et al. Naringenin inhibits autophagy and epithelial-mesenchymal transition of human lens epithelial cells by regulating the Smad2/3 pathway. Drug Dev Res 83:389-396 (2022). PubMed: 34402084
- Spurlin JW et al. BMP3 inhibits TGFβ2-mediated myofibroblast differentiation during wound healing of the embryonic cornea. NPJ Regen Med 7:36 (2022). PubMed: 35879352
- Jie X et al. USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGF-ß2 transcription. Cell Death Differ 28:2095-2111 (2021). PubMed: 33558705
- Finger AM et al. Intercellular coupling between peripheral circadian oscillators by TGF-ß signaling. Sci Adv 7:N/A (2021). PubMed: 34301601
- Corbet C et al. TGFß2-induced formation of lipid droplets supports acidosis-driven EMT and the metastatic spreading of cancer cells. Nat Commun 11:454 (2020). PubMed: 31974393
- Babushkina A & Lwigale P Periocular neural crest cell differentiation into corneal endothelium is influenced by signals in the nascent corneal environment. Dev Biol 465:119-129 (2020). PubMed: 32697973
- Tran DQ et al. GARP (LRRC32) is essential for the surface expression of latent TGF-beta on platelets and activated FOXP3+ regulatory T cells. Proc Natl Acad Sci U S A 106:13445-50 (2009). PubMed: 19651619
- O'Riordan E et al. Chronic NOS inhibition actuates endothelial-mesenchymal transformation. Am J Physiol Heart Circ Physiol 292:H285-94 (2007). PubMed: 16963618