Active human TGF beta 2 full length protein (ab84070)

Overview

Description

  • NatureRecombinant
  • SourceHEK 293 cells
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceALDAAYCFRN VQDNCCLRPL YIDFKRDLGW KWIHEPKGYN ANFCAGACPY LWSSDTQHSR VLSLYNTINP EASASPCCVS QDLEPLTILY YIGKTPKIEQ LSNMIVKSCK CS
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab84070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityBiological Activity : Determined by its ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The ED50 was found to be = 0.2 ng/ml, corresponding to a specific activity of = 5 x 106 units/mg.
  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Purity> 95 % by SDS-PAGE.
    Purity is greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Store at -20°C. Stable for 12 months at -20°C

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstituted TGF beta 2 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.

General info

  • Alternative names
    • BSC-1 cell growth inhibitor
    • BSC1 cell growth inhibitor
    • Cetermin
    • G-TSF
    • Glioblastoma-derived T-cell suppressor factor
    • GTSF
    • MGC116892
    • MGF
    • Milk growth factor
    • Polyergin
    • TGF-beta-2
    • TGF-beta2
    • TGFB2
    • TGFB2_HUMAN
    • Transforming growth factor beta 2
    • Transforming growth factor beta-2
    see all
  • FunctionTGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.
  • Involvement in diseaseNote=A chromosomal aberration involving TGFB2 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with HDAC9.
    Defects in TGFB2 are the cause of Loeys-Dietz syndrome 4 (LDS4) [MIM:614816]. An aortic aneurysm syndrome with widespread systemic involvement. LDS4 is characterized by arterial tortuosity, aortic dissection, intracranial aneurysm and subarachnoid hemorrhage, hypertelorism, bifid uvula, pectus deformity, bicuspid aortic valve, arachnodactyly, scoliosis, foot deformities, dural ectasia, joint hyperflexibility, and thin skin with easy bruising and striae.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P61812 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Active human TGF beta 2 full length protein images

  • Anti-TGF beta 2 antibody (ab66045) at 1 µg/ml + Active human TGF beta 2 full length protein (ab84070) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Active human TGF beta 2 full length protein (ab84070)

This product has been referenced in:
  • Tran DQ  et al. GARP (LRRC32) is essential for the surface expression of latent TGF-beta on platelets and activated FOXP3+ regulatory T cells. Proc Natl Acad Sci U S A 106:13445-50 (2009). Read more (PubMed: 19651619) »
  • O'Riordan E  et al. Chronic NOS inhibition actuates endothelial-mesenchymal transformation. Am J Physiol Heart Circ Physiol 292:H285-94 (2007). Read more (PubMed: 16963618) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. Reconstitution instructions for the proteins are below.

Centrifuge the vial prior to opening. Reconstitute in 10mM Citric Acid, pH 3.0 to a
concentration of 0.1-1.0 mg/ml. Do not vortex. For
extended storag...

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Thank you for contacting us.

I can confirm that the product can be reconstituted with sterile water at conc. 0.1-1mg/ml.

Please note the concentration will depend on the amount of water you add. e.g. if 1ml is added the conc. will be ...

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Thank you for contacting Abcam regarding ab66045.


I am sorry that the caption describing the WB image was confusing. Please allow me to clarify:


The image represents a WB in which the antibody ab66045 was used to detect ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"