Active human WISP2 full length protein (ab50040)

Overview

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMQLCPTPCTC PWPPPRCPLG VPLVLDGCGC CRVCARRLGE PCDQLHVCDA SQGLVCQPGA GPGGRGALCL LAEDDSSCEV NGRLYREGET FQPHCSIRCR CEDGGFTCVP LCSEDVRLPS WDCPHPRRVE VLGKCCPEWV CGQGGGLGTQ PLPAQGPQFS GLVSSLPPGV PCPEWSTAWG PCSTTCGLGM ATRVSNQNRF CRLETQRRLC LSRPCPPSRG RSPQNSAF
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab50040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    Functional Studies

  • Purity> 95 % by SDS-PAGE.
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Note: Do not raise to neutral pH. Do not vortex. This solution can be stored at 2-8oC for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC.

General info

  • Alternative names
    • CCN family member 5
    • CCN5
    • Connective tissue growth factor like protein
    • Connective tissue growth factor related protein 58
    • Connective tissue growth factor-like protein
    • Connective tissue growth factor-related protein 58
    • CT58
    • CTGF L
    • CTGF-L
    • WISP 2
    • WISP-2
    • Wisp2
    • WISP2_HUMAN
    • Wnt 1 signaling pathway protein 2
    • WNT1 inducible signaling pathway protein 2
    • WNT1-inducible-signaling pathway protein 2
    see all
  • FunctionMay play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.
  • Tissue specificityExpressed in primary osteoblasts, fibroblasts, ovary, testes, and heart.
  • Sequence similaritiesBelongs to the CCN family.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession O76076 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Active human WISP2 full length protein images

  • Anti-WISP2 antibody (ab38317) at 1/100 dilution + Active human WISP2 full length protein (ab50040) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Active human WISP2 full length protein (ab50040)

ab50040 has not yet been referenced specifically in any publications.

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