Active Listeriolysin (LLO) protein fragment (ab68200)

Overview

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • Amino acids60 to 529

Specifications

Our Abpromise guarantee covers the use of ab68200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityActivity : 1.25x106 HU/mg. 2 mM DTT could be use to reactivate the toxin.
  • Applications

    SDS-PAGE

  • Purity> 90 % by SDS-PAGE.
    Purity: Greater than 90.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE
  • FormLiquid
  • Additional Notes


    This protein can be used for: Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 5% Glycerol, 0.5M Sodium chloride, 50mM Sodium dihydrogen phosphate, 2.7mM Potassium chloride, 1mM DTT, 1mM EDTA

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General info

  • Alternative names
    • hly
    • hlyA
    • lisA
    • LLO
    • Thiol activated cytolysin
    see all
  • RelevanceListeria monocyogenes is a faculative intracellular Gram-positive food borne bacterium, increasingly recognized as being responsible for severe infections in both humans and animals. Ingestion of contaminated food causes an infection called Listeriosis, which affects especially immunocomprimised patients, new borns and pregnant women. It is characterized by severe syndromes such as encephalitis, meningoencephalitis, septicemia and abortion. Listeriolysin O (LLO) is a single polypeptide protein secreted by the Gram-positive bacterium Listeria monocytogenes. LLO belongs to the group of cholesterol-binding sulfhydrylactivated toxins, the lytic activity of which is enhanced by reducing agents and is suppressed by exposure to oxygen or cholesterol. LLO hemolytic activity is maximum at pH 5.5 and rapidly decreases with the increase of the pH.

References for Active Listeriolysin (LLO) protein fragment (ab68200)

ab68200 has not yet been referenced specifically in any publications.

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