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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
Our Abpromise guarantee covers the use of ab792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 0.1M MOP. pH 4.0-4.5
This product is an active protein and may elicit a biological response in vivo, handle with caution.
MBP and Biotinylated MBP was phosphorylated with 0.05ug of active ERK1 with P32 radiolabeled ATP.
Assay reference: Sanghera JS et al., J Immunol. (1996) 156:4457-4465
Activity of biotinylated MBP
Lane a (5 ug of biotinylated MBP): 33682 counts/min
Lane b (2.5 ug of biotinylated MBP): 18478 counts/min
Lane c (1.25 ug of biotinylated MBP): 6714 counts/min
Lane d (0.625 ug of biotinylated MBP): 2889 counts/min
Lane e (0.36 ug of biotinylated MBP): 1039 counts/min
Lane f (0.18 ug of biotinylated MBP): 633 counts/min
Control Lane (0.0 ug of biotinylated MBP): 145 counts/min
Non-biotinylated MBP Activity
Lane A (5 ug of biotinylated MBP): 37604 counts/min
Lane B (2.5 ug of biotinylated MBP): 17824 counts/min
Lane C (1.25 ug of biotinylated MBP): 8867 counts/min
Lane D (0.625 ug of biotinylated MBP): 4190 counts/min
Lane E (0.36 ug of biotinylated MBP): 2248 counts/min
Lane F (0.18 ug of biotinylated MBP): 1500 counts/min
Control Lane (0.0 ug of biotinylated MBP): 218 counts/min
ab792 has not yet been referenced specifically in any publications.
Publishing research using ab792 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"