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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
Directly conjugated antibodies for immunofluorescence
Our Abpromise guarantee covers the use of ab792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 0.1M MOP. pH 4.0-4.5
This product is an active protein and may elicit a biological response in vivo, handle with caution.
MBP and Biotinylated MBP was phosphorylated with 0.05ug of active ERK1 with P32 radiolabeled ATP.
Assay reference: Sanghera JS et al., J Immunol. (1996) 156:4457-4465
Activity of biotinylated MBP
Lane a (5 ug of biotinylated MBP): 33682 counts/min
Lane b (2.5 ug of biotinylated MBP): 18478 counts/min
Lane c (1.25 ug of biotinylated MBP): 6714 counts/min
Lane d (0.625 ug of biotinylated MBP): 2889 counts/min
Lane e (0.36 ug of biotinylated MBP): 1039 counts/min
Lane f (0.18 ug of biotinylated MBP): 633 counts/min
Control Lane (0.0 ug of biotinylated MBP): 145 counts/min
Non-biotinylated MBP Activity
Lane A (5 ug of biotinylated MBP): 37604 counts/min
Lane B (2.5 ug of biotinylated MBP): 17824 counts/min
Lane C (1.25 ug of biotinylated MBP): 8867 counts/min
Lane D (0.625 ug of biotinylated MBP): 4190 counts/min
Lane E (0.36 ug of biotinylated MBP): 2248 counts/min
Lane F (0.18 ug of biotinylated MBP): 1500 counts/min
Control Lane (0.0 ug of biotinylated MBP): 218 counts/min
ab792 has not yet been referenced specifically in any publications.
Publishing research using ab792 ? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"