Active Proteinase K full length protein (ab51501)

Overview

  • Product nameActive Proteinase K full length protein
  • DescriptionActive Proteinase K full length protein

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab51501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    In Situ Hybridisation

    ChIP

    SDS-PAGE

  • FormLyophilised
  • Additional NotesWarning: This product is irritating to eyes, respiratory system and skin, and may cause sensitization by inhalation.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute with 1ml of 50mM Tris/HCI buffer (pH7.6).

General info

  • Alternative names
    • Endopeptidase K
    • Tritirachium alkaline proteinase
  • RelevanceProteinase K is an endolytic protease that cleaves peptide bonds preferentially next to the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids. Proteinase K is classified as a serine protease and exhibits broad substrate specificity. It can hydrolyze keratin; hence the name. Proteinase K is used for the digestion of proteins on tissue sections as a pre-treatment to aid in the preparation of mRNA and its detection by in situ hybridisation methods using oligonucleotide probes.

Protocols

References for Active Proteinase K full length protein (ab51501)

ab51501 has not yet been referenced specifically in any publications.

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