Anti-Activin A antibody [MM0074-7L18] (ab89307)

Overview

  • Product name
    Anti-Activin A antibody [MM0074-7L18]
  • Description
    Mouse monoclonal [MM0074-7L18] to Activin A
  • Specificity
    Detects both pro- and mature Activin A.
  • Tested applications
    Suitable for: WB, Neutralisingmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Activin A. Human recombinant Pro-Activin-A
    Database link: P08476

Properties

Applications

Our Abpromise guarantee covers the use of ab89307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 56 kDa.
Neutralising Use at an assay dependent dilution. (1) Activin A can induce hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. (2) Anti-Activin antibody can neutralize Activin A induced hemoglobin expression in a dose dependent manner. ED 50 is approximately between 30-60ng/mL in the presence of 5ng/mL Recombinant Human Activin A.

Target

  • Function
    Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Form
    Activin A is a homodimer of beta-A.
  • Alternative names
    • Activin A antibody
    • Activin beta A chain antibody
    • Activin beta-A chain antibody
    • ActivinA antibody
    • EDF antibody
    • Erythroid differentiation factor antibody
    • Erythroid differentiation protein antibody
    • INHBA antibody
    • INHBA_HUMAN antibody
    • Inhibin beta A chain antibody
    see all

Anti-Activin A antibody [MM0074-7L18] images

  • All lanes : Anti-Activin A antibody [MM0074-7L18] (ab89307) at 1 µg/ml

    Lane 1 : Embryonic Day 18 Liver
    Lane 2 : Embryonic Day 18 Muscles
    Lane 3 : Embryonic Day 18 Cortex (Negative Control)

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa


    Exposure time : 2 minutes

    This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

    See Abreview

References for Anti-Activin A antibody [MM0074-7L18] (ab89307)

This product has been referenced in:
  • Tsigkou A  et al. Expression Levels of Myostatin and Matrix Metalloproteinase 14 mRNAs in Uterine Leiomyoma are Correlated With Dysmenorrhea. Reprod Sci 22:1597-602 (2015). Read more (PubMed: 26138721) »
  • Karve TM  et al. BRCA1 regulates follistatin function in ovarian cancer and human ovarian surface epithelial cells. PLoS One 7:e37697 (2012). WB ; Human . Read more (PubMed: 22685544) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rhesus monkey Cell lysate - whole cell (PBMC)
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine)
Loading amount
30 µg
Treatment
1µg/mL E. coli LPS for 24hr (10^6 cells/mL)
Specification
PBMC
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Alan Curtis

Verified customer

Submitted Jul 26 2016

Thank you for contacting us. I contacted the lab in order to get as many information about the Anti-Activin antibody [MM0074-7L18] (ab89307) as possible for you.


It is known that


(1) Activin A can induce haemoglobin expressi...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human glioblastoma cell line)
Specification
human glioblastoma cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - Tritonx100 0.1% in PBS
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Apr 29 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (liver, muscle, brain)
Loading amount
20 µg
Specification
liver, muscle, brain
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 07 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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