Anti-Activin A antibody [MM0074-7L18] (ab89307)


  • Product nameAnti-Activin A antibody [MM0074-7L18]
  • Description
    Mouse monoclonal [MM0074-7L18] to Activin A
  • SpecificityDetects both pro- and mature Activin A.
  • Tested applicationsSuitable for: WB, Neutralisingmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Activin A. Human recombinant Pro-Activin-A
    Database link: P08476



Our Abpromise guarantee covers the use of ab89307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 56 kDa.
Neutralising Use at an assay dependent dilution. (1) Activin A can induce hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. (2) Anti-Activin antibody can neutralize Activin A induced hemoglobin expression in a dose dependent manner. ED 50 is approximately between 30-60ng/mL in the presence of 5ng/mL Recombinant Human Activin A.


  • FunctionInhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • FormActivin A is a homodimer of beta-A.
  • Alternative names
    • Activin A antibody
    • Activin beta A chain antibody
    • Activin beta-A chain antibody
    • ActivinA antibody
    • EDF antibody
    • Erythroid differentiation factor antibody
    • Erythroid differentiation protein antibody
    • INHBA antibody
    • INHBA_HUMAN antibody
    • Inhibin beta A chain antibody
    see all

Anti-Activin A antibody [MM0074-7L18] images

  • All lanes : Anti-Activin A antibody [MM0074-7L18] (ab89307) at 1 µg/ml

    Lane 1 : Embryonic Day 18 Liver
    Lane 2 : Embryonic Day 18 Muscles
    Lane 3 : Embryonic Day 18 Cortex (Negative Control)

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa

    Exposure time : 2 minutes

    This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

    See Abreview

References for Anti-Activin A antibody [MM0074-7L18] (ab89307)

This product has been referenced in:
  • Karve TM  et al. BRCA1 regulates follistatin function in ovarian cancer and human ovarian surface epithelial cells. PLoS One 7:e37697 (2012). WB ; Human . Read more (PubMed: 22685544) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rhesus monkey Cell lysate - whole cell (PBMC)
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine)
Loading amount 30 µg
Treatment 1µg/mL E. coli LPS for 24hr (10^6 cells/mL)
Specification PBMC
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Alan Curtis

Verified customer

Submitted Jul 26 2016

Thank you for contacting us. I contacted the lab in order to get as many information about the Anti-Activin antibody [MM0074-7L18] (ab89307) as possible for you.

It is known that

(1) Activin A can induce haemoglobin expressi...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line)
Specification human glioblastoma cell line
Fixative Paraformaldehyde
Permeabilization Yes - Tritonx100 0.1% in PBS
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 29 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (liver, muscle, brain)
Loading amount 20 µg
Specification liver, muscle, brain
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 07 2010