Anti-Activin A Receptor Type IB antibody (ab10594)

Overview

  • Product name
    Anti-Activin A Receptor Type IB antibody
    See all Activin A Receptor Type IB primary antibodies
  • Description
    Goat polyclonal to Activin A Receptor Type IB
  • Specificity
    Based on immunoblotting, this antibody exhibits approximately 2 % cross-reactivity with recombinant human activin receptor IIA and recombinant human activin receptor IA and less than 1 % cross-reactivity with recombinant human activin receptor IIA and recombinant human activin receptor IIB.
  • Tested applications
    Suitable for: IHC-P, IHC-FoFr, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    purified recombinant human activin receptor IB extracellular domain expressed in mouse NSO cells.

  • Positive control
    • human breast carcinoma
  • General notes


    Activin, a disulfide-linked homodimeric protein is secreted by Sertoli cells in the testis and granulosa cells in the ovary. In early studies, this peptide was thought to be an inhibin and not recognized as a unique compound. Activins and inhibins are members of the TGF-beta superfamily due to amino acid homology with respect to the conservation of 7 of the 9 cysteine residues common to all TGF-beta forms. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits have been cloned (mammalian betaA, betaB, betaC, betaE, and Xenopus betaD). The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A (betaA-betaA), activin B (betaB-betaB), activin AB (betaB-betaA), inhibin A (alpha-betaA), and inhibin B (alpha- betaB). Activins have a wide range of biological activities including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis, and reproductive physiology. Activins are also involved in growth and differentiation of several tissues from different species. This protein also plays a key role in the production and regulation of hormones such as FSH, LH, GnRH, and ACTH. Activin influences erythropoiesis and the potentiation of erythroid colony formation, oxytocin secretion, paracrine, and autocrine regulation. Similar to other TGF-beta family members, activins exert their biological activities through the effects ot the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated type I and type receptors. Activin type I and type II receptors are distinguished by the level of sequence homology of their kinase domains and other structural and functional features. To date, seven type I and five type II activin receptors have been cloned from mammals, including activin receptor IA, activin receptor IIA, activin receptor IB, and activin receptor IIB. In addition, two splice variants of activin receptor IIA and five splice variants of activin receptor IIB have been reported. Type I activin receptors are highly conserved and do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Type I activin receptors will also bind with the BMP-2/7-bound BMPR-II and form signaling complexes. Human, mouse, and bovine type IB activin receptors share greater than 98 % homology.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Activin, a disulfide-linked homodimeric protein is secreted by Sertoli cells in the testis and granulosa cells in the ovary. In early studies, this peptide was thought to be an inhibin and not recognized as a unique compound. Activins and inhibins are members of the TGF-beta superfamily due to amino acid homology with respect to the conservation of 7 of the 9 cysteine residues common to all TGF-beta forms. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits have been cloned (mammalian betaA, betaB, betaC, betaE, and Xenopus betaD). The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A (betaA-betaA), activin B (betaB-betaB), activin AB (betaB-betaA), inhibin A (alpha-betaA), and inhibin B (alpha- betaB). Activins have a wide range of biological activities including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis, and reproductive physiology. Activins are also involved in growth and differentiation of several tissues from different species. This protein also plays a key role in the production and regulation of hormones such as FSH, LH, GnRH, and ACTH. Activin influences erythropoiesis and the potentiation of erythroid colony formation, oxytocin secretion, paracrine, and autocrine regulation. Similar to other TGF-beta family members, activins exert their biological activities through the effects ot the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated type I and type receptors. Activin type I and type II receptors are distinguished by the level of sequence homology of their kinase domains and other structural and functional features. To date, seven type I and five type II activin receptors have been cloned from mammals, including activin receptor IA, activin receptor IIA, activin receptor IB, and activin receptor IIB. In addition, two splice variants of activin receptor IIA and five splice variants of activin receptor IIB have been reported. Type I activin receptors are highly conserved and do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Type I activin receptors will also bind with the BMP-2/7-bound BMPR-II and form signaling complexes. Human, mouse, and bovine type IB activin receptors share greater than 98 % homology.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-FoFr Use a concentration of 5 - 15 µg/ml.

Use ABC amplification.

WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 57 kDa.

Detects human activin receptor IB at approximately 5 ng/lane under reducing and non-reducing conditions.

ELISA Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function
    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Phosphorylates TDP2.
  • Tissue specificity
    Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 GS domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Form
    There are 3 isoforms produced by alternative splicing. Isoform 1 also known as SKR2-1; isoform 2 as SKR2-2; isoform 3 as SKR2-3.
  • Alternative names
    • Activin A receptor type 1B antibody
    • Activin A receptor type II like kinase 4 antibody
    • Activin A type 1B receptor antibody
    • Activin A type IB receptor antibody
    • Activin receptor like kinase 4 antibody
    • Activin receptor type 1B antibody
    • Activin receptor type IB antibody
    • Activin receptor type-1B antibody
    • Activin receptor-like kinase 4 antibody
    • ACTR IB antibody
    • ACTR-IB antibody
    • ACTRIB antibody
    • ACV1B_HUMAN antibody
    • ACVR 1B antibody
    • Acvr1b antibody
    • ACVRLK 4 antibody
    • ACVRLK4 antibody
    • ALK 4 antibody
    • ALK-4 antibody
    • ALK4 antibody
    • Serine(threonine) protein kinase receptor R2 antibody
    • Serine/threonine-protein kinase receptor R2 antibody
    • SKR 2 antibody
    • SKR2 antibody
    see all

Images

  • Human breast carcinoma tissue stained with ab10594 (5ug/ml). See recommended protocol for further details.

References

ab10594 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast)
Specification
Breast
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
No
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted May 12 2009

We have just received a detailed protocol from the source of ab10594 who has tested the antibody in IHC on breast carcinoma tissue. I enclose the protocol and hope this will help you. If you are still not satisfied with the product please let me kno...

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