Anti-ALK-7 antibody (ab111121)
Key features and details
- Rabbit polyclonal to ALK-7
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-ALK-7 antibody
See all ALK-7 primary antibodies -
Description
Rabbit polyclonal to ALK-7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human ALK-7 (internal sequence).
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Positive control
- ICC/IF: COS7 cells. IHC-P: Human brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.88% Sodium chloride, PBS
PBS is without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab111121 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/500.
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Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Serine/threonine protein kinase which forms a receptor complex on ligand binding. The receptor complex consisting of 2 type II and 2 type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators, SMAD2 and SMAD3. Receptor for activin AB, activin B and NODAL. Plays a role in cell differentiation, growth arrest and apoptosis. -
Tissue specificity
Present in pancreas, heart, colon, small intestine, ovary and the hippocampus, medulla oblongata and putamen of the brain. Isoform 1, isoform 2, isoform 3 and isoform 4 are all expressed in the placenta throughout pregnancy. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 130399 Human
- Entrez Gene: 269275 Mouse
- Entrez Gene: 245921 Rat
- Omim: 608981 Human
- SwissProt: Q8NER5 Human
- SwissProt: Q8K348 Mouse
- SwissProt: P70539 Rat
- Unigene: 562901 Human
see all -
Alternative names
- Activin A receptor, type IC antibody
- Activin receptor like kinase 7 antibody
- Activin receptor type 1C precursor antibody
see all
Images
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Immunofluorescence analysis of ALK-7 in COS7 cells, using ab111121 at a 1/100 dilution. The imge on the right is from cells treated with the synthesized peptide.
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ab111121, at a 1/50 dilution, staining ALK-7 in paraffin embedded human brain tissue by Immunohistochemistry. The image on the right is from cells treated with the synthesized peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab111121 has been referenced in 4 publications.
- Hong X et al. Biological effects of NODAL on endometrial cancer cells and its underlying mechanisms. Exp Ther Med 21:402 (2021). PubMed: 33717261
- Shi Q et al. Knockdown of ALK7 inhibits high glucose-induced oxidative stress and apoptosis in retinal pigment epithelial cells. Clin Exp Pharmacol Physiol 47:313-321 (2020). PubMed: 31608496
- Tengroth L et al. Activation of Activin receptor-like kinases curbs mucosal inflammation and proliferation in chronic rhinosinusitis with nasal polyps. Sci Rep 8:1561 (2018). PubMed: 29367682
- Li H et al. Nodal induced by hypoxia exposure contributes to dacarbazine resistance and the maintenance of stemness in melanoma cancer stem-like cells. Oncol Rep 39:2855-2864 (2018). PubMed: 29693169