Anti-Activin antibody [MM0074-7L18] (ab89307)

Overview

  • Product nameAnti-Activin antibody [MM0074-7L18]
  • Description
    Mouse monoclonal [MM0074-7L18] to Activin
  • SpecificityDetects both pro- and mature Activin A.
  • Tested applicationsWB, Neutralising more details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length Human Precursor Activin A

Properties

Applications

Our Abpromise guarantee covers the use of ab89307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/1000. Predicted molecular weight: 56 kDa.
Neutralising Use at an assay dependent dilution. (1) Activin A can induce hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. (2) Anti-Activin antibody can neutralize Activin A induced hemoglobin expression in a dose dependent manner. ED 50 is approximately between 30-60ng/mL in the presence of 5ng/mL Recombinant Human Activin A.

Target

  • FunctionInhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P08476 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Activin A antibody
    • Activin AB alpha polypeptide antibody
    • Activin AB antibody
    • Activin AB beta polypeptide antibody
    • Activin B antibody
    • Activin beta A chain antibody
    • Activin beta B chain antibody
    • Activin beta-A chain antibody
    • ActivinA antibody
    • ActivinAB antibody
    • ActivinB antibody
    • EDF antibody
    • Erythroid differentiation factor antibody
    • Erythroid differentiation protein antibody
    • follicle stimulating hormone releasing protein antibody
    • FRP antibody
    • FSH releasing factor antibody
    • FSH releasing protein antibody
    • INHBA antibody
    • INHBA_HUMAN antibody
    • INHBB antibody
    • Inhibin antibody
    • Inhibin beta 1 antibody
    • Inhibin beta 2 antibody
    • Inhibin beta A antibody
    • Inhibin beta A chain antibody
    • Inhibin beta A subunit antibody
    • Inhibin beta B antibody
    • Inhibin beta B chain antibody
    • Inhibin beta B subunit antibody
    • Inhibin, beta A (activin A, activin AB alpha polypeptide) antibody
    see all

Anti-Activin antibody [MM0074-7L18] images

  • All lanes : Anti-Activin antibody [MM0074-7L18] (ab89307) at 1 µg/ml

    Lane 1 : Embryonic Day 18 Liver
    Lane 2 : Embryonic Day 18 Muscles
    Lane 3 : Embryonic Day 18 Cortex (Negative Control)

    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa


    Exposure time : 2 minutes

    Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

    See Abreview

References for Anti-Activin antibody [MM0074-7L18] (ab89307)

This product has been referenced in:
  • Karve TM  et al. BRCA1 regulates follistatin function in ovarian cancer and human ovarian surface epithelial cells. PLoS One 7:e37697 (2012). WB ; Human . Read more (PubMed: 22685544) »

See 1 Publication for this product

Product Wall

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It is known that


(1) Activin A can induce haemoglobin expressi...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line)
Specification human glioblastoma cell line
Fixative Paraformaldehyde
Permeabilization Yes - Tritonx100 0.1% in PBS
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Apr 29 2011

Application Western blot
Sample Rat Tissue lysate - whole (liver, muscle, brain)
Loading amount 20 µg
Specification liver, muscle, brain
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 07 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"