Anti-Activin Receptor Type IIA antibody (ab96793)

Overview

  • Product nameAnti-Activin Receptor Type IIA antibody
    See all Activin Receptor Type IIA primary antibodies
  • Description
    Rabbit polyclonal to Activin Receptor Type IIA
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 267 and 509 of Human Activin receptor type IIA (AAH69707).

  • Positive control
    • HeLa or HepG2 cell lysate This antibody gave a positive result in IF/ICC when used in the following formaldehyde fixed cell lines: DU145.

Properties

Applications

Our Abpromise guarantee covers the use of ab96793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 58 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activin A receptor type IIA antibody
    • Activin receptor type 2A antibody
    • Activin receptor type IIA antibody
    • Activin receptor type-2A antibody
    • ACTR 2 antibody
    • ACTR IIA antibody
    • ACTR-IIA antibody
    • ACTR2 antibody
    • ACTRII antibody
    • ACTRIIA antibody
    • Acvr 2 antibody
    • Acvr 2A antibody
    • Acvr2 antibody
    • ACVR2A antibody
    • AVR2A_HUMAN antibody
    • OTTHUMP00000197918 antibody
    see all

Anti-Activin Receptor Type IIA antibody images

  • All lanes : Anti-Activin Receptor Type IIA antibody (ab96793) at 1/10000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : Hep G2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 58 kDa
  • ICC/IF image of ab96793 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96793 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Activin Receptor Type IIA antibody (ab96793)

ab96793 has not yet been referenced specifically in any publications.

Product Wall

Thank you for calling Abcam earlier today.

As we discussed, I am sending you ab18130 free of charge so that you can confirm the western blot results that you are seeing with ab96793.

If there is anything else I can help you with, pl...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"