Anti-Activin Receptor Type IIB antibody (ab76940)

Overview

  • Product nameAnti-Activin Receptor Type IIB antibody
    See all Activin Receptor Type IIB primary antibodies
  • Description
    Mouse monoclonal to Activin Receptor Type IIB
  • Tested applicationsSuitable for: WB, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Macaque Monkey
  • Immunogen

    Recombinant fragment with proprietary tag: RGEAETRECI YYNANWELER TNQSGLERCE GEQDKRLHCY ASWANSSGTI ELVKKGCWLD DFNCYDRQEC VATEENPQVY FCCCEGNFCN ERFTHLPEAG, corresponding to amino acids 21-120 of Human Activin Receptor Type IIB(NP_001097)

  • Positive control
    • Lysate from 293T cells transfected with Activin Receptor Type IIB

Properties

Applications

Our Abpromise guarantee covers the use of ab76940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 58 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A.
  • Involvement in diseaseDefects in ACVR2B are the cause of visceral heterotaxy autosomal type 4 (HTX4) [MIM:613751]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy type 4 include dextrocardia, right aortic arch and a right-sided spleen, anomalies of the inferior and the superior vena cava, atrial ventricular canal defect with dextro-transposed great arteries, pulmonary stenosis, polysplenia and midline liver.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activin A receptor type IIB antibody
    • Activin receptor type 2B antibody
    • Activin receptor type IIB antibody
    • Activin receptor type-2B antibody
    • ActR IIB antibody
    • ACTR-IIB antibody
    • ActRIIB antibody
    • ACVR 2B antibody
    • ACVR2B antibody
    • AVR2B_HUMAN antibody
    • HTX4 antibody
    • MGC116908 antibody
    see all

Anti-Activin Receptor Type IIB antibody images

  • Anti-Activin Receptor Type IIB antibody (ab76940) at 1/500 dilution + immunogen (GST-tagged 100aa recombinant fragment) at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

    Predicted band size : 58 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HeLa cells stained with ab76940 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76940, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • All lanes : Anti-Activin Receptor Type IIB antibody (ab76940) at 1/500 dilution

    Lane 1 : Activin Receptor Type IIB transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 58 kDa

References for Anti-Activin Receptor Type IIB antibody (ab76940)

This product has been referenced in:
  • Perron JC & Dodd J Inductive specification and axonal orientation of spinal neurons mediated by divergent bone morphogenetic protein signaling pathways. Neural Dev 6:36 (2011). Read more (PubMed: 22085733) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Odyssey Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Sample Human Cell (LNCaP Human Cell Line)
Specification LNCaP Human Cell Line
Permeabilization Yes - Ethanol
Fixative Paraformaldehyde
Username

Dr. FRANCESCO ELIA MARINO

Verified customer

Submitted Mar 25 2015

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (12)
Sample Mouse Tissue lysate - whole (Testis)
Specification Testis
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Abcam user community

Verified customer

Submitted Oct 21 2014

Merci de nous avoir contactés.

Suite à notre conversation du 7 Novembre, j’ai demandé au laboratoire des explications pour les images du western blot pour ab76940. Puisque les deux images étaient contradicto...

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Suite à notre conversation téléphonique je vais demander au laboratoire des explications pour les images de western blot pour ab76940. En attendant leur réponse, je vous envoie le code pour l’offre de test pour ab12854...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (C2C12 mouse myoblast and C57 muscle extract)
Loading amount 40 µg
Specification C2C12 mouse myoblast and C57 muscle extract
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Thomas Roberts

Verified customer

Submitted Jul 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"