Overview

  • Product nameAnti-ACTL8 antibody
    See all ACTL8 primary antibodies
  • Description
    Rabbit polyclonal to ACTL8
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 156 and 353 of ACTL8 (NP_110439)

  • Positive control
    • A431 and Molt-4 whole cell lysate. H1299, HeLa, HepG2 cell lines. A549 xenograft tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.
IHC-P 1/100 - 1/500. Antigen retrieval using heat mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0) is suggested.

Target

  • Tissue specificityStrongly expressed in testis and pancreas. Weak expression in placenta.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin like 8 antibody
    • Actin like protein 8 antibody
    • Actin like protein antibody
    • Actin-like protein 8 antibody
    • ACTL8 antibody
    • ACTL8_HUMAN antibody
    • Cancer/testis antigen 57 antibody
    • CT57 antibody
    see all

Anti-ACTL8 antibody images

  • All lanes : Anti-ACTL8 antibody (ab96756) at 1/1000 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : Molt-4 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 41 kDa
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft , using ab96756 at 1/500 dilution.
  • ab96756 at 1/200 staining ACTL8 in methanol-fixed HeLa by Immunofluorescence, lower image is costained with Hoeschst 33342.

References for Anti-ACTL8 antibody (ab96756)

ab96756 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab96756.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"