Overview

  • Product name
  • Description
    Rabbit polyclonal to ACTL8
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 156 and 353 of ACTL8 (NP_110439)

  • Positive control
    • A431 and Molt-4 whole cell lysate. H1299, HeLa, HepG2 cell lines. A549 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.
IHC-P 1/100 - 1/500. Antigen retrieval using heat mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0) is suggested.

Target

  • Tissue specificity
    Strongly expressed in testis and pancreas. Weak expression in placenta.
  • Sequence similarities
    Belongs to the actin family.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin like 8 antibody
    • Actin like protein 8 antibody
    • Actin like protein antibody
    • Actin-like protein 8 antibody
    • ACTL8 antibody
    • ACTL8_HUMAN antibody
    • Cancer/testis antigen 57 antibody
    • CT57 antibody
    see all

Images

  • All lanes : Anti-ACTL8 antibody (ab96756) at 1/1000 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : Molt-4 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 41 kDa
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft , using ab96756 at 1/500 dilution.
  • ab96756 at 1/200 staining ACTL8 in methanol-fixed HeLa by Immunofluorescence, lower image is costained with Hoeschst 33342.

References

ab96756 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab96756.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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