Overview

  • Product name
  • Description
    Rabbit polyclonal to ACTN3
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide from the N-terminus of Human ACTN3, conjugated to KLH.

  • Positive control
    • Human skeletal muscle tissue, HT-29 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab113234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Predicted molecular weight: 103 kDa.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function
    F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
  • Tissue specificity
    Expressed only in a subset of type 2 skeletal muscle fibers.
  • Sequence similarities
    Belongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actinin alpha 3 antibody
    • actinin, alpha antibody
    • ACTN 3 antibody
    • ACTN3 antibody
    • ACTN3_HUMAN antibody
    • Alpha actinin skeletal muscle antibody
    • Alpha actinin skeletal muscle isoform 3 antibody
    • Alpha-Actinin 3 antibody
    • Alpha-actinin skeletal muscle isoform 3 antibody
    • Alpha-actinin-3 antibody
    • F actin cross linking protein antibody
    • F-actin cross-linking protein antibody
    • MGC117002 antibody
    • MGC117005 antibody
    • OTTHUMP00000219049 antibody
    • OTTHUMP00000219054 antibody
    see all

Images

  • ab113234 at 10 µg/ml staining ACTN3 in formalin-fixed, paraffin-embedded Human skeletal muscle tissue.
  • All lanes : Anti-ACTN3 antibody (ab113234) at 1/500 dilution

    Lane 1 : Extracts from HT-29 cells
    Lane 2 : Extracts from HT-29 cells with treated with synthetic peptide


    Predicted band size : 103 kDa

References

ab113234 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your question regarding antibody ab113234. We have not tested ab113234 for cross reactivity with other family members experimentally. A BLAST search comparing the immunogenic peptide to the sequences below yields the following homology ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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