Anti-ACTN3 antibody [EP2531Y] (ab68204)

Overview

  • Product nameAnti-ACTN3 antibody [EP2531Y]
    See all ACTN3 primary antibodies
  • Description
    Rabbit monoclonal [EP2531Y] to ACTN3
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues on the N-terminus of human ACTN3.

  • Positive control
    • Human skeletal muscle lysate and human muscle tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab68204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/5000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
    • Tissue specificityExpressed only in a subset of type 2 skeletal muscle fibers.
    • Sequence similaritiesBelongs to the alpha-actinin family.
      Contains 1 actin-binding domain.
      Contains 2 CH (calponin-homology) domains.
      Contains 2 EF-hand domains.
      Contains 4 spectrin repeats.
    • Information by UniProt
    • Database links
    • Alternative names
      • Actinin alpha 3 antibody
      • actinin, alpha antibody
      • ACTN 3 antibody
      • ACTN3 antibody
      • ACTN3_HUMAN antibody
      • Alpha actinin skeletal muscle antibody
      • Alpha actinin skeletal muscle isoform 3 antibody
      • Alpha-Actinin 3 antibody
      • Alpha-actinin skeletal muscle isoform 3 antibody
      • Alpha-actinin-3 antibody
      • F actin cross linking protein antibody
      • F-actin cross-linking protein antibody
      • MGC117002 antibody
      • MGC117005 antibody
      • OTTHUMP00000219049 antibody
      • OTTHUMP00000219054 antibody
      see all

    Anti-ACTN3 antibody [EP2531Y] images

    • Anti-ACTN3 antibody [EP2531Y] (ab68204) at 1/10000 dilution + human skeletal muscle lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 103 kDa
      Observed band size : 103 kDa
    • ab68204 staining ACTN3 in human pluripotent stem cell derived cardiomyocyte by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 5% serum for 15 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • Immunohistochemical analysis of paraffin-embedded human muscle tissue using ab68204 at 1/100-1/250 dilution.

    References for Anti-ACTN3 antibody [EP2531Y] (ab68204)

    ab68204 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Heart)
    Permeabilization Yes - Tween-20
    Specification Heart
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 22 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Cell (skeletal muscle/frozen sections, human, rat and mo)
    Permeabilization Yes - 0,1 % Triton X-100 in PBS
    Specification skeletal muscle/frozen sections, human, rat and mo
    Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative Histofix/Histolab/Sweden
    Username

    Dr. Satu Koskinen

    Verified customer

    Submitted Aug 01 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Skeletal Muscle)
    Gel Running Conditions Reduced Denaturing (4%-12%)
    Loading amount 10 µg
    Specification Skeletal Muscle
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 25 2016

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Human pluripotent stem cell derived cardiomyocyte)
    Permeabilization Yes - saponin
    Specification Human pluripotent stem cell derived cardiomyocyte
    Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Apr 21 2015

    Thank you for contacting us.
    Skeletal Muscle (Human) Tissue Lysate is recommended as a positive control for ab68204. Both products are recommended for Western Blotting, and are guaranteed to work in this application.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"