Purification notesWhole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIF: 1/100.
For indirect immunofluorescence, a minimum working antibody dilution of 1:100 is recommended using mouse fibroblasts NIH3T cell.
WB: 1/1000 (recommended using a rat brain cytosol extract and a whole cell extract of the human epidermoid carcinoma A431 cell line). Predicted molecular weight: 45 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome.
Sequence similaritiesBelongs to the actin family. ARP1 subfamily.