Overview

  • Product name
  • Description
    Rabbit polyclonal to ACTR1A
  • Specificity
    The antibody is specific to a peptide sequence highly conserved (89% identity) in the alpha centractin isoform, and not found in known actin isoforms.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide:

    ACYLSINPQKDETLETEKAQ

    conjugated to KLH, corresponding to amino acids 222-240 of Human ACTR1A.

  • General notes
    If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab11009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    IF: 1/100.
    For indirect immunofluorescence, a minimum working antibody dilution of 1:100 is recommended using mouse fibroblasts NIH3T cell.
    WB: 1/1000 (recommended using a rat brain cytosol extract and a whole cell extract of the human epidermoid carcinoma A431 cell line). Predicted molecular weight: 45 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Component of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome.
    • Sequence similarities
      Belongs to the actin family. ARP1 subfamily.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Actin related protein 1 antibody
      • Actin RPV antibody
      • Actin-RPV antibody
      • ACTR 1A antibody
      • Actr1a antibody
      • ACTZ_HUMAN antibody
      • Alpha centractin antibody
      • Alpha-centractin antibody
      • ARP 1 antibody
      • ARP1 actin related protein 1 homolog A antibody
      • ARP1 actin related protein 1 homolog A centractin alpha antibody
      • ARP1 antibody
      • ARP1 yeast homolog A antibody
      • Centractin alpha antibody
      • Centractin antibody
      • Centrosome associated actin homolog antibody
      • Centrosome-associated actin homolog antibody
      • CTRN 1 antibody
      • CTRN1 antibody
      • FLJ52695 antibody
      • FLJ52800 antibody
      • FLJ55002 antibody
      see all

    Images

    • Immunohistochemistry (Formalin fixed, paraffin-embedded sections) analysis of rat duodenum tissue labelling ACTR1A with ab11009.

    • Immunohistochemistry (Formalin fixed, paraffin-embedded sections) analysis of rat kidney tissue labelling ACTR1A with ab11009.

    • All lanes : Anti-ACTR1A antibody (ab11009) at 1/1000 dilution

      Lane 1 : A432 cell lysate
      Lane 2 : A432 cell lysate with ACTR1A immunising peptide at 5 mg/ml


      Predicted band size : 45 kDa
      Observed band size : 45 kDa

    References

    ab11009 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Cow Cell lysate - whole cell (Lung)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 26 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    African green monkey Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 26 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 26 2017

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 26 2017

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African green monkey Cell (Kidney)
    Permeabilization
    Yes - 0.2% triton x 100
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jun 15 2017

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.3% triton x 100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 22 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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