Overview

  • Product nameAnti-AcV5 tag antibody [AcV5]
  • Description
    Mouse monoclonal [AcV5] to AcV5 tag
  • SpecificityThis antibody recognizes a nine amino acid residue tag (SWKDASGWS) of the baculovirus Autographa californica GP64 envelope fusion protein. Proteins tagged with this AcV5 peptide are functional in both dicots and monocots plants and show no cross reactivity across many plant species.
  • Tested applicationsSuitable for: IP, ICC, Dot blotmore details
  • Species reactivity
    Reacts with
  • Immunogen

    AcNPV extracellular nonocculeded virus (NOV)

  • Positive control
    • Extracts of Sf9 cells infected with baculovirus.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • ClonalityMonoclonal
  • Clone numberAcV5
  • MyelomaSp2/0-Ag14
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
Dot blot Use a concentration of 0.5 - 1 µg/ml.

Target

  • RelevanceEpitope tagged fusion proteins such as AcV5 tag are widely used in protein analysis of biological samples (e.g. plant cell extracts). Antibodies specific for the tag are a valuable means for the detection of the protein. AcV5 recognizes a nine amino acid residue tag of baculovirus Autographa californica GP64 envelope fusion protein (efp). GP64 gene open reading frame encodes a protein of 509 amino acids with a molecular weight of 58 kDa. The protein contains N-linked glycosylation sites and hydrophobic N- and C-termini, characteristic of signal and membrane anchor motifs found in envelope glycoproteins. The GP64 protein is present early (6 hr) post infection and accumulates in the infected cell moving to the periphery later.

References for Anti-AcV5 tag antibody [AcV5] (ab49581)

ab49581 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"