Overview

  • Product name
    Anti-Acylglycerol Kinase antibody
    See all Acylglycerol Kinase primary antibodies
  • Description
    Goat polyclonal to Acylglycerol Kinase
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    CDPRKREQMLTSP

    , corresponding to C terminal amino acids 408-420 of Human Acylglycerol Kinase (NP_060708.1).

  • Positive control
    • Lysate from Human Brain (Substantia nigra, Hippocampus)

Properties

Applications

Our Abpromise guarantee covers the use of ab77266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/16000.
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth.
    • Tissue specificity
      Highly expressed in muscle, heart, kidney and brain.
    • Pathway
      Lipid metabolism; glycerolipid metabolism.
    • Sequence similarities
      Contains 1 DAGKc domain.
    • Cellular localization
      Mitochondrion membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2610037M15Rik antibody
      • 6720408I04Rik antibody
      • Acylglycerol kinase mitochondrial antibody
      • Acylglycerol kinase, mitochondrial antibody
      • agk antibody
      • AGK_HUMAN antibody
      • AI465370 antibody
      • FLJ10842 antibody
      • hAGK antibody
      • HsMuLK antibody
      • MuLK antibody
      • Multi substrate lipid kinase antibody
      • Multi-substrate lipid kinase antibody
      • Multiple substrate lipid kinase antibody
      • RGD1562046 antibody
      see all

    Anti-Acylglycerol Kinase antibody images

    • Anti-Acylglycerol Kinase antibody (ab77266) at 1 µg/ml + Human Brain (Substantia nigra) lysate in RIPA buffer at 35 µg

      Predicted band size : 47 kDa
      Observed band size : 47 kDa
      Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-Acylglycerol Kinase antibody (ab77266)

    This product has been referenced in:
    • Abu El-Asrar AM  et al. Expression of bioactive lysophospholipids and processing enzymes in the vitreous from patients with proliferative diabetic retinopathy. Lipids Health Dis 13:187 (2014). Read more (PubMed: 25496321) »
    • Abu El-Asrar AM  et al. Expression of lysophosphatidic acid, autotaxin and acylglycerol kinase as biomarkers in diabetic retinopathy. Acta Diabetol 50:363-71 (2013). Read more (PubMed: 22864860) »

    See all 2 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab77266.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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