Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCatalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.
Tissue specificityFound in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues.
Involvement in diseaseDefects in ADA are the cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-negative due to adenosine deaminase deficiency (ADASCID) [MIM:102700]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. ADA-SCID is an autosomal recessive form accounting for about 50% of non-X-linked SCIDs. ADA deficiency has been diagnosed in chronically ill teenagers and adults (late or adult onset). Population and newborn screening programs have also identified several healthy individuals with normal immunity who have partial ADA deficiency.
Sequence similaritiesBelongs to the adenosine and AMP deaminases family.
Cellular localizationCell membrane. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion.
Immunohistochemistry (PFA perfusion fixed frozen sections) - ADA antibody (ab77756)
ab77756, at 1/250 dilution, staining ADA in Rat superior colliculus by Immunohistochemistry of 4.0% paraformaldehyde fixed tissue.
- ADA antibody (ab77756)Image kindly supplied by Mr Johannes De Jong through Abreview
ab77756 staining ADA in rat brain tissue by Immunohistochemistry (4% PFA perfused, free floating sections). Samples were incubated with the primary antibody at a 1/250 dilution for 16 hours at 23°C. A donkey anti-rabbit IgG conjugated tp Cy3® was used as secondary antibody at a 1/200 dilution.