• Product nameAnti-ADA antibody (Biotin)
    See all ADA primary antibodies
  • Description
    Rabbit polyclonal to ADA (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Flow Cyt, Dot Blot, ELISA, Immunomicroscopymore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Adenosine Deaminase from Calf spleen.

  • General notes

    10-20 Biotin molecules per rabbit IgG molecule.



Our Abpromise guarantee covers the use of ab34677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. (PMID 20147632)

ab200208-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Dot Blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

This antibody has been assayed against 1.0µg of Adenosine Deaminase in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.

Immunomicroscopy Use at an assay dependent concentration.


  • FunctionCatalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.
  • Tissue specificityFound in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues.
  • Involvement in diseaseDefects in ADA are the cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-negative due to adenosine deaminase deficiency (ADASCID) [MIM:102700]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. ADA-SCID is an autosomal recessive form accounting for about 50% of non-X-linked SCIDs. ADA deficiency has been diagnosed in chronically ill teenagers and adults (late or adult onset). Population and newborn screening programs have also identified several healthy individuals with normal immunity who have partial ADA deficiency.
  • Sequence similaritiesBelongs to the adenosine and AMP deaminases family.
  • Cellular localizationCell membrane. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion.
  • Information by UniProt
  • Database links
  • Alternative names
    • ada antibody
    • ADA_HUMAN antibody
    • ADA1 antibody
    • Adenosine aminohydrolase antibody
    • Adenosine deaminase antibody
    see all

References for Anti-ADA antibody (Biotin) (ab34677)

This product has been referenced in:
  • Chou JP  et al. Accelerated aging in HIV/AIDS: novel biomarkers of senescent human CD8+ T cells. PLoS One 8:e64702 (2013). Flow Cyt ; Human . Read more (PubMed: 23717651) »
  • Parish ST  et al. Adenosine deaminase modulation of telomerase activity and replicative senescence in human CD8 T lymphocytes. J Immunol 184:2847-54 (2010). Flow Cyt ; Human . Read more (PubMed: 20147632) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry and your interest in our products. According to the datasheets, ab54969 has not been tested for ELISA (only WB, Flow Cytmetry); whilst the other antibody (ab34677) has been characterized for indirect ELISA system. Unfor...

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Thank you for your patience while I investigated the source of the note on our datasheet that specified the molecular weight of ADA as 104 kDa. The predicted molecular weight should be given as 40.7 kDa, based on the UniProt database entry: ...

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