Overview

  • Product nameAnti-ADA2a antibody
    See all ADA2a primary antibodies
  • Description
    Rabbit polyclonal to ADA2a
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide: EYKSALLNEC NKQGGLRLAQ ARALIKIDVN KTRKIYDFLI REGYITKG, corresponding to internal sequence amino acids 396-443 of Human ADA2a (NP_001479)

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab85657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceMany DNA binding transcriptional activator proteins enhance the initiation rate of RNA polymerase II mediated gene transcription by interacting functionally with the general transcription machinery bound at the basal promoter. Adaptor proteins are usually required for this activation, possibly to acetylate and destabilize nucleosomes, thereby relieving chromatin constraints at the promoter. ADA2a is a transcriptional activator adaptor and has been found to be part of the PCAF histone acetylase complex.
  • Cellular localizationNuclear, Chromosome
  • Database links
  • Alternative names
    • ADA2 ALPHA antibody
    • ADA2 antibody
    • ADA2 like antibody
    • ADA2 like protein antibody
    • ADA2, yeast, homolog of, alpha antibody
    • ADA2A antibody
    • hADA2 antibody
    • KL04P antibody
    • Tada2l antibody
    • Transcriptional adapter 2 alpha antibody
    • Transcriptional adapter 2 like antibody
    • Transcriptional adaptor 2 (ADA2 homolog, yeast) like antibody
    • Transcriptional adaptor 2 (ADA2, yeast, homolog) like antibody
    • Transcriptional adaptor 2 alpha antibody
    • Transcriptional adaptor 2A antibody
    see all

Anti-ADA2a antibody images

  • Anti-ADA2a antibody (ab85657) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

References for Anti-ADA2a antibody (ab85657)

ab85657 has not yet been referenced specifically in any publications.

Product Wall

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Based on the information from Uniprot regarding the Ada2a protein, there are two isoforms of the protein:

http://www.uniprot.org/uniprot/O75478



One isoform is 36kDa and the other ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"