Overview

  • Product name
  • Description
    Rabbit polyclonal to ADAM10
  • Tested applications
    Suitable for: IHC-P, WB, ICC, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human ADAM10 aa 732-748.
    Sequence:

    PQRQRPRESYQMGHMRR


    (Peptide available as ab7868)

  • Positive control
    • WB: Jurkat whole cell lysate. ICC: K562 cells. ICC/IF: HeLa and K562 cells.
  • General notes
    TNFa converting enzyme.

Properties

Applications

Our Abpromise guarantee covers the use of ab1997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500.
WB 1/500 - 1/2000. Predicted molecular weight: 84 kDa.Can be blocked with ADAM10 peptide (ab7868).
ICC Use a concentration of 2 µg/ml.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 2 µg/ml.

Target

  • Function
    Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
    -Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis.
  • Tissue specificity
    Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
  • Sequence similarities
    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization
    Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloprotease domain 10 antibody
    • A disintegrin and metalloproteinase domain 10 antibody
    • AD 10 antibody
    • AD10 antibody
    • AD18 antibody
    • ADA10_HUMAN antibody
    • ADAM 10 antibody
    • ADAM metallopeptidase domain 10 antibody
    • ADAM10 antibody
    • CD 156c antibody
    • CD156c antibody
    • CD156c antigen antibody
    • CDw156 antibody
    • disintegrin and metalloproteinase domain containing protein 10 antibody
    • Disintegrin and metalloproteinase domain-containing protein 10 antibody
    • HsT 18717 antibody
    • HsT18717 antibody
    • Kuz antibody
    • Kuzbanian antibody
    • Kuzbanian protein homolog antibody
    • Kuzbanian, Drosophila, homolog of antibody
    • MADM antibody
    • Mammalian disintegrin metalloprotease antibody
    • Mammalian disintegrin-metalloprotease antibody
    • RAK antibody
    see all

Anti-ADAM10 antibody images

  • Lane 1 : Anti-ADAM10 antibody (ab1997) at 1 µg/ml
    Lane 2 : Anti-ADAM10 antibody (ab1997) at 2 µg/ml

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : Jurkat whole cell lysate


    Predicted band size : 84 kDa
  • ab1997 at 2µg/ml staining K562 cells by ICC/IF.
  • ICC/IF image of ab1997 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1997, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-ADAM10 antibody (ab1997) at 1/1666 dilution

    Lane 2 : ADAM10 siRNA treated human keratinocyte cells
    Lane 3 : Non-treated human keratinocyte cells.

    Secondary
    HRP conjugated Goat anti-rabbit antibody
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 75,100 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Immunofluorescence of ADAM10 in K562 cells using ab1997 at 10 ug/ml.

  • All lanes : Anti-ADAM10 antibody (ab1997) at 1/1000 dilution

    Lane 1 : Human frontal cortex tissue lysate - whole
    Lane 2 : Human frontal cortex tissue lysate - whole

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye® 800CW-conjugated donkey anti-rabbit IgG polyclonal at 1/18000 dilution

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 65,80 kDa (why is the actual band size different from the predicted?)


    Exposure time : 330 seconds

    This image is courtesy of an Abreview submitted by Grant Corbett

    See Abreview

References for Anti-ADAM10 antibody (ab1997)

This product has been referenced in:
  • Lu Y  et al. Regulated intramembrane proteolysis of the AXL receptor kinase generates an intracellular domain that localizes in the nucleus of cancer cells. FASEB J 31:1382-1397 (2017). WB . Read more (PubMed: 28034848) »
  • Jiao T  et al. Role of MicroRNA-103a Targeting ADAM10 in Abdominal Aortic Aneurysm. Biomed Res Int 2017:9645874 (2017). WB, IHC ; Mouse . Read more (PubMed: 28357407) »

See all 23 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (4L melanoma cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
4L melanoma cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Praveen Agrawal

Verified customer

Submitted Mar 24 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kidney)
Permeabilization
Yes - 0.1% troton x-100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 06 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 08 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Loading amount
40 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 08 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 02 2016

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Melanoma cell line)
Total protein in input
1000 µg
Immuno-precipitation step
Protein G
Specification
Melanoma cell line
Username

Dr. Praveen Agrawal

Verified customer

Submitted Nov 18 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen and oesophagus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Spleen and oesophagus
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 23 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Sample
Mouse Cell (Neurons)
Specification
Neurons
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Nov 04 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8% in MES)
Sample
Human Tissue lysate - whole (Frontal Cortex)
Specification
Frontal Cortex
Blocking step
Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Grant Corbett

Verified customer

Submitted Jul 18 2014

Thank you very much for your call today and for your patience.

I apologize for the delay getting this protocol to you, as it needed to be typed up and re-formatted before sending. Please find the protocol used to test the antibody in ELISA at...

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