Overview

  • Product name
  • Description
    Rabbit polyclonal to ADAM10
  • Specificity
    This antibody is specific for the furin cleavage site of ADAM10, and can detect the latent form of the enzyme.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide based on the prohormone convertase (PC) cleavage site of human ADAM10. (Peptide available as ab41166.)

Properties

Applications

Our Abpromise guarantee covers the use of ab39180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 10 µg/ml.
    WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects a band of approximately 98 kDa due to glycosylation and the cyteine-rich regions and 58-60 when Furin processed. (predicted molecular weight: 84 kDa). EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.


    Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
      -Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis.
    • Tissue specificity
      Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
    • Sequence similarities
      Contains 1 disintegrin domain.
      Contains 1 peptidase M12B domain.
    • Domain
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications
      The precursor is cleaved by a furin endopeptidase.
    • Cellular localization
      Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
    • Information by UniProt
    • Database links
    • Alternative names
      • A disintegrin and metalloprotease domain 10 antibody
      • A disintegrin and metalloproteinase domain 10 antibody
      • AD 10 antibody
      • AD10 antibody
      • AD18 antibody
      • ADA10_HUMAN antibody
      • ADAM 10 antibody
      • ADAM metallopeptidase domain 10 antibody
      • ADAM10 antibody
      • CD 156c antibody
      • CD156c antibody
      • CD156c antigen antibody
      • CDw156 antibody
      • disintegrin and metalloproteinase domain containing protein 10 antibody
      • Disintegrin and metalloproteinase domain-containing protein 10 antibody
      • HsT 18717 antibody
      • HsT18717 antibody
      • Kuz antibody
      • Kuzbanian antibody
      • Kuzbanian protein homolog antibody
      • Kuzbanian, Drosophila, homolog of antibody
      • MADM antibody
      • Mammalian disintegrin metalloprotease antibody
      • Mammalian disintegrin-metalloprotease antibody
      • RAK antibody
      see all

    Images

    • Methanol fixed HeLa cells were incubated with ab39180 (10 µg/ml) for 1 hour at 22°C. For further details please refer to the Abreview.

    • Anti-ADAM10 antibody (ab39180) at 1/2000 dilution + whole cell lysate prepared from EL4 cells at 100000 cells

      Secondary
      HRP conjugated donkey anti-rabbit polyclonal at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 84 kDa
      Observed band size : 60,80 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      Image courtesy of an anonymous Abreview.

      See Abreview

    References

    This product has been referenced in:
    • Doi K  et al. Crucial role of the Rap G protein signal in Notch activation and leukemogenicity of T-cell acute lymphoblastic leukemia. Sci Rep 5:7978 (2015). Read more (PubMed: 25613394) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (RERF-LC-Ad1 cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    RERF-LC-Ad1 cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 06 2017

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (EL4)
    Loading amount
    100000 cells
    Specification
    EL4
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted May 11 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Hela Cells)
    Specification
    Hela Cells
    Fixative
    Methanol
    Permeabilization
    No
    Username

    Abcam user community

    Verified customer

    Submitted Feb 25 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela Cells)
    Loading amount
    60000 cells
    Specification
    Hela Cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 18 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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