Anti-ADAM10 antibody - Aminoterminal end (ab39153)

Overview

  • Product nameAnti-ADAM10 antibody - Aminoterminal end
    See all ADAM10 primary antibodies
  • Description
    Rabbit polyclonal to ADAM10 - Aminoterminal end
  • SpecificityThis antibody recognizes the amino end of active ADAM10, and can detect the latent and active forms of the enzyme.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide of Human ADAM10 based on the prohormone convertase (PC) cleavage site. (Peptide available as ab41167.)

Properties

Applications

Our Abpromise guarantee covers the use of ab39153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa).

Target

  • FunctionCleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
    -Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis.
  • Tissue specificityExpressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
  • Sequence similaritiesContains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localizationCell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloprotease domain 10 antibody
    • A disintegrin and metalloproteinase domain 10 antibody
    • AD 10 antibody
    • AD10 antibody
    • ADA10_HUMAN antibody
    • ADAM 10 antibody
    • ADAM metallopeptidase domain 10 antibody
    • ADAM10 antibody
    • CD 156c antibody
    • CD156c antibody
    • CD156c antigen antibody
    • CDw156 antibody
    • disintegrin and metalloproteinase domain containing protein 10 antibody
    • Disintegrin and metalloproteinase domain-containing protein 10 antibody
    • HsT 18717 antibody
    • HsT18717 antibody
    • Kuz antibody
    • Kuzbanian antibody
    • Kuzbanian protein homolog antibody
    • MADM antibody
    • Mammalian disintegrin metalloprotease antibody
    • Mammalian disintegrin-metalloprotease antibody
    see all

Anti-ADAM10 antibody - Aminoterminal end images

  • IHC image of ab39153 staining in human normal lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39153, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-ADAM10 antibody - Aminoterminal end (ab39153)

This product has been referenced in:
  • Xiao Q  et al. Functional role of matrix metalloproteinase-8 in stem/progenitor cell migration and their recruitment into atherosclerotic lesions. Circ Res 112:35-47 (2013). WB ; Mouse . Read more (PubMed: 23071158) »
  • Epis R  et al. Blocking ADAM10 synaptic trafficking generates a model of sporadic Alzheimer's disease. Brain 133:3323-35 (2010). WB ; Mouse . Read more (PubMed: 20805102) »

See all 2 Publications for this product

Product Wall

Thank you for your inquiry.

Yes, you are correct. If we supply the antibody at 1 mg/ml and you need to use it at 5 ug/ml that is a 1/200 dilution. 1 mg/ml x ml/.005 mg = 200

If you want to put .005 mL in 1 mL, this would be 1/200. <...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela Cells)
Loading amount 60000 cells
Specification Hela Cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted Oct 30 2007

Thank you for your enquiry and your interest in our products. As I have mentioned in the previous mail, the information you requested is confidential and we have no access to you. However, after check with our source, I can confirm that the epitope ...

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Thank you for your enquiry. The antibodies are >95% pure by SDS-PAGE. The sodium chloride concentration is 0.25 M. The phosphate concentration has changed slightly over the course of years as the peptide-affinity column elution protocol has been opti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"