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Products:Cell Biology >> Apoptosis >> Receptors >> Receptor Processing
Overview
Product name
Anti-ADAM10 antibody - Cytoplasmic domain
See all ADAM10 products (10) ...
Description
Rabbit polyclonal to ADAM10 - Cytoplasmic domain
Specificity
This antibody is specific for the Cytoplasmic domain of ADAM10 and can detect latent and active forms of the enzyme.It will not recognize the shorter form, lacking the transmembrane and Cytoplasmic domains.
Tested applications
IHC-FoFr, IHC-P, WB, ICC/IF, IPmore details
Cross reactivity
Reacts with
Mouse, Cow, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide based on the Cytoplasmic domain of human ADAM10, within the last 30 C-terminus amino acids of the 748 amino acid human sequence.
(Peptide available as ab41164.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Cell Biology >> Apoptosis >> Receptors >> Receptor Processing
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent concentration. (PubMed: 21325636)
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB: 1/1000 - 1/5000.Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa).
ICC/IF: Use a concentration of 10 µg/ml
IP: Use at an assay dependent concentration. (PubMed: 19823572Bovine ADAM10-HA construct)
Target
Function
Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis.
Tissue specificity
Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
Target information above from: UniProt accessionO14672
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A disintegrin and metalloprotease domain 10 antibody
- A Disintegrin And Metalloproteinase Domain 10 antibody
- A Disintegrin And Metalloproteinase Domain 10 antibody
see all
Database links
- Entrez Gene: 282132 Cow
- Entrez Gene: 102 Human
- Entrez Gene: 11487 Mouse
- Entrez Gene: 29650 Rat
- Omim: 602192 Human
- SwissProt: Q10741 Cow
- SwissProt: O14672 Human
- SwissProt: O35598 Mouse
see all
Anti-ADAM10 antibody - Cytoplasmic domain images:
Immunocytochemistry/ Immunofluorescence - ADAM10 antibody - Cytoplasmic domain (ab39177)
Methanol fixed HeLs cells stained with ab39177 (10 µg/ml) for 1 hour at 22°C. For further details please refer to the Abreview
Image taken from anonymous abreview published in February 2008
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAM10 antibody - Cytoplasmic domain (ab39177)
IHC image of ab39177 staining in human normal lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39177, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References for Anti-ADAM10 antibody - Cytoplasmic domain (ab39177)
This product has been referenced in:
- Cong Ret al. A disintegrin and metalloprotease 10 activity sheds the ectodomain of the amyloid precursor-like protein 2 and regulates protein expression in proximal tubule cells. Am J Physiol Cell Physiol 300:C1366-74 (2011). WB, IHC-FoFr; Mouse.Read more (PubMed: 21325636) »
- Shirakabe Ket al. VIP36 Protein Is a Target of Ectodomain Shedding and Regulates Phagocytosis in Macrophage Raw 264.7 Cells. J Biol Chem 286:43154-63 (2011). WB; Mouse.Read more (PubMed: 22016386) »
See all 4 publications for this product
Publishing research using ab39177? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"