| | | | Immunocytochemistry/ Immunofluorescence - ADAM10 antibody - Cytoplasmic domain (ab39177)  (enlarge) | |
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| Product Name | ADAM10 antibody - Cytoplasmic domain |
| | See all ADAM10 antibodies (8)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to ADAM10 - Cytoplasmic domain |
| Immunogen | Synthetic peptide based on the Cytoplasmic domain of human ADAM10, within the last 30 C-terminus amino acids of the 748 amino acid human sequence.
(Peptide available as ab41164.) |
| Reacts with | Hu |
| Predicted to react (species key) | Ms, Rat (Due to sequence homology) |
| Specificity | This antibody is specific for the Cytoplasmic domain of ADAM10 and can detect latent and active forms of the enzyme.It will not recognize the shorter form, lacking the transmembrane and Cytoplasmic domains. We have a range of domain specific antibodies for this target. For a full list please see all ADAM10 antibodies |
| Tested applications (see key) | ICC/IF, WB |
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| Abreviews | |
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| Application notes (see key) | Recommended dilutions ICC/IF: Use at a concentration of 10 µg/ml. WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects a band of approximately 98 kDa and 58-60 kDa when Furin processed. (predicted molecular weight: 84 kDa). EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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| Cellular localization | Cell membrane, Type I membrane protein. Also localized in the Golgi apparatus.
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| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family Cell Biology >> Apoptosis >> Receptors >> Receptor Processing |
| Relevance | ADAM10 is a member of the ADAM (a disintegrin and metalloprotease like domain) family. It cleaves the membrane-bound precursor of TNF-alpha at 76-Ala-|-Val-77 to its mature soluble form and is responsible for the proteolytic release of several cell-surface proteins, including heparin binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). It contributes to the normal cleavage of the cellular prion protein and is involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicules, suggesting a vesicle-based protease activity. |
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| Database links | The links below go to external sites and will open in a new browser window |
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.05% Sodium Azide Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product: Glycerol MSDS Sodium Azide MSDS
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| Purification notes | The antibody has been peptide-affinity purified. |
| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. |
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| See below for ADAM10 antibody - Cytoplasmic domain images, references, products related to ab39177 and other tools. |
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ADAM10 antibody - Cytoplasmic domain images: |
Immunocytochemistry/ Immunofluorescence - ADAM10 antibody - Cytoplasmic domain (ab39177) Methanol fixed HeLs cells stained with ab39177 (10 µg/ml) for 1 hour at 22°C. For further details please refer to the Abreview Image taken from anonymous abreview published in February 2008 |
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| Search PubMed (MEDLINE) for references to ADAM10 |
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