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Synthetic peptide based on the Cytoplasmic domain of human ADAM10, within the last 30 C-terminus amino acids of the 748 amino acid human sequence.
(Peptide available as ab41164.)
Our Abpromise guarantee covers the use of ab39177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 21325636|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa).Can be blocked with ADAM10 peptide (ab41164).|
|ICC/IF||Use a concentration of 10 µg/ml.|
|IP||Use at an assay dependent concentration. PubMed: 19823572Bovine ADAM10-HA construct|
Methanol fixed HeLs cells stained with ab39177 (10 µg/ml) for 1 hour at 22°C. For further details please refer to the Abreview
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"