ADAM10 antibody - Cytoplasmic domain (ab39177)

Product Name 

ADAM10 antibody - Cytoplasmic domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to ADAM10 - Cytoplasmic domain

Immunogen 

Synthetic peptide based on the Cytoplasmic domain of human ADAM10, within the last 30 C-terminus amino acids of the 748 amino acid human sequence.

(Peptide available as ab41164.)

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat
(Due to sequence homology)

Specificity 

This antibody is specific for the Cytoplasmic domain of ADAM10 and can detect latent and active forms of the enzyme.It will not recognize the shorter form, lacking the transmembrane and Cytoplasmic domains.

We have a range of domain specific antibodies for this target. For a full list please see all ADAM10 antibodies

Tested applications 
(see key)

ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects a band of approximately 98 kDa and 58-60 kDa when Furin processed. (predicted molecular weight: 84 kDa). EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell membrane, Type I membrane protein. Also localized in the Golgi apparatus.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ADAMs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> ADAM protein family
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> ADAM Protein Family
Cell Biology >> Apoptosis >> Receptors >> Receptor Processing

Relevance 

ADAM10 is a member of the ADAM (a disintegrin and metalloprotease like domain) family. It cleaves the membrane-bound precursor of TNF-alpha at 76-Ala-|-Val-77 to its mature soluble form and is responsible for the proteolytic release of several cell-surface proteins, including heparin binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). It contributes to the normal cleavage of the cellular prion protein and is involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicules, suggesting a vesicle-based protease activity.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody has been peptide-affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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