You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-ADAM10 antibody - Cytoplasmic domain (ab39177)

CodeSizePriceAbpointsAvailability
    
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-ADAM10 antibody - Cytoplasmic domain
See all ADAM10 products (10) ...

Description

Rabbit polyclonal to ADAM10 - Cytoplasmic domain

Specificity

This antibody is specific for the Cytoplasmic domain of ADAM10 and can detect latent and active forms of the enzyme.It will not recognize the shorter form, lacking the transmembrane and Cytoplasmic domains.

Tested applications

IHC-FoFr, IHC-P, WB, ICC/IF, IPmore details

Cross reactivity

Reacts with

Mouse, Cow, Human

Predicted to work with

Rat

Immunogen

Synthetic peptide based on the Cytoplasmic domain of human ADAM10, within the last 30 C-terminus amino acids of the 748 amino acid human sequence.

(Peptide available as ab41164.)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody has been peptide-affinity purified.

Clonality

Polyclonal

Isotype

IgG

  • Immunocytochemistry/ Immunofluorescence - ADAM10 antibody - Cytoplasmic domain (ab39177)Immunocytochemistry/ Immunofluorescence - ADAM10 antibody - Cytoplasmic domain (ab39177) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAM10 antibody - Cytoplasmic domain (ab39177)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAM10 antibody - Cytoplasmic domain (ab39177) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab39177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis.

Tissue specificity

Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.

Sequence similarities

Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.

Domain

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Post-translational
modifications

The precursor is cleaved by a furin endopeptidase.

Cellular localization

Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.

Target information above from: UniProt accessionO14672 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • A disintegrin and metalloprotease domain 10 antibody
  • A Disintegrin And Metalloproteinase Domain 10 antibody
  • A Disintegrin And Metalloproteinase Domain 10 antibody
  • AD 10 antibody
  • AD10 antibody
  • ADA10_HUMAN antibody
  • ADAM 10 antibody
  • ADAM metallopeptidase domain 10 antibody
  • ADAM10 antibody
  • CD 156c antibody
  • CD156c antibody
  • CD156c antigen antibody
  • CDw156 antibody
  • disintegrin and metalloproteinase domain containing protein 10 antibody
  • Disintegrin and metalloproteinase domain-containing protein 10 antibody
  • HsT 18717 antibody
  • HsT18717 antibody
  • kuz antibody
  • Kuz antibody
  • Kuzbanian antibody
  • Kuzbanian antibody
  • Kuzbanian protein homolog antibody
  • MADM antibody
  • MADM antibody
  • Mammalian disintegrin metalloprotease antibody
  • Mammalian disintegrin-metalloprotease antibody
see all

Anti-ADAM10 antibody - Cytoplasmic domain images:

  Immunocytochemistry/ Immunofluorescence - ADAM10 antibody - Cytoplasmic domain (ab39177)

Immunocytochemistry/ Immunofluorescence - ADAM10 antibody - Cytoplasmic domain (ab39177)

Methanol fixed HeLs cells stained with ab39177 (10 µg/ml) for 1 hour at 22°C. For further details please refer to the Abreview

Image taken from anonymous abreview published in February 2008

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAM10 antibody - Cytoplasmic domain (ab39177)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADAM10 antibody - Cytoplasmic domain (ab39177)

IHC image of ab39177 staining in human normal lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39177, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-ADAM10 antibody - Cytoplasmic domain (ab39177)

This product has been referenced in:

  • Cong Ret al. A disintegrin and metalloprotease 10 activity sheds the ectodomain of the amyloid precursor-like protein 2 and regulates protein expression in proximal tubule cells. Am J Physiol Cell Physiol 300:C1366-74 (2011). WB, IHC-FoFr; Mouse.Read more (PubMed: 21325636) »
  • Shirakabe Ket al. VIP36 Protein Is a Target of Ectodomain Shedding and Regulates Phagocytosis in Macrophage Raw 264.7 Cells. J Biol Chem 286:43154-63 (2011). WB; Mouse.Read more (PubMed: 22016386) »

See all 4 publications for this product

Publishing research using ab39177? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"