Overview

  • Product nameAnti-ADAM12 antibody
    See all ADAM12 primary antibodies
  • Description
    Goat polyclonal to ADAM12
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    AARPLPVSPARAL

    with a C terminal Cysteine residue linker, corresponding to N terminal amino acids 2-14 of Human ADAM12 (NP_003465.3).

  • Positive control
    • Human placenta tissue; Human heart lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab115203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 100 kDa.
ELISA 1/64000.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionInvolved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
  • Tissue specificityIsoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
  • Sequence similaritiesContains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • DomainThe cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase domain 12 antibody
    • ADA12_HUMAN antibody
    • ADAM 12 antibody
    • ADAM metallopeptidase domain 12 antibody
    • ADAM12 antibody
    • Disintegrin and metalloproteinase domain-containing protein 12 antibody
    • MCMP antibody
    • MCMPMltna antibody
    • Meltrin alpha antibody
    • Meltrin-alpha antibody
    • MLTN antibody
    • MLTNA antibody
    • OTTHUMP00000046766 antibody
    see all

Anti-ADAM12 antibody images

  • ab115203 at 3.75 µg/ml staining ADAM12 in Formalin-fixed, Paraffin-embedded Human placenta tissue by Immunohistochemistry.
  • Anti-ADAM12 antibody (ab115203) at 0.1 µg/ml + Human heart lysate (in RIPA buffer) at 30 µg
    Developed using the ECL technique

    Predicted band size : 100 kDa

References for Anti-ADAM12 antibody (ab115203)

ab115203 has not yet been referenced specifically in any publications.

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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No problem, I will send the product ab56366.

Could you let me know the purchase order number of ab39155?

Thanks!

Thank you for your email.

Lab has confirmed that the immunogen for ab115203 and ab28747 is indeed corresponds to N-terminal end and is in signal peptide region, so these antibodies might not be suitable.

Regarding ab56366 this antibod...

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I have requested confirmation of immunogen sequence from lab. I will get back to you with the reply soon. Once it gets confirmed I will then be able to suggest the suitable antibody.

Many thanks for having patience.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"