SpecificityThis antibody is expected to recognise both the longer membrane bound form of human ADAM12 and the shorter soluble ADAM12 splice isoform.
This antibody does not cross react with other ADAMS.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 100 kDa).
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. PubMed: 19213876
FunctionInvolved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
Tissue specificityIsoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
DomainThe cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading. The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational modificationsThe precursor is cleaved by a furin endopeptidase.
ab28747 at 3.8µg/ml staining ADAM12 in human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval using citrate pH 6 was performed. AP staining used as detection method.
References for Anti-ADAM12 antibody (ab28747)
This product has been referenced in:
Estrella C et al. Role of A disintegrin and metalloprotease-12 in neutrophil recruitment induced by airway epithelium. Am J Respir Cell Mol Biol41:449-58 (2009).
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