Overview

  • Product nameAnti-ADAM12 antibody
    See all ADAM12 primary antibodies
  • Description
    Mouse monoclonal to ADAM12
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: ETLKATKYVE LVIVADNREF QRQGKDLEKV KQRLIEIANH VDKFYRPLNI RIVLVGVEVW NDMDKCSVSQ DPFTSLHEFL DWRKMKLLPR KSHDNAQ, corresponding to amino acids 208-305 of Human ADAM12

Properties

Applications

Our Abpromise guarantee covers the use of ab56366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
    • Tissue specificityIsoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
    • Sequence similaritiesContains 1 disintegrin domain.
      Contains 1 EGF-like domain.
      Contains 1 peptidase M12B domain.
    • DomainThe cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications
      The precursor is cleaved by a furin endopeptidase.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • A disintegrin and metalloproteinase domain 12 antibody
      • ADA12_HUMAN antibody
      • ADAM 12 antibody
      • ADAM metallopeptidase domain 12 antibody
      • ADAM12 antibody
      • Disintegrin and metalloproteinase domain-containing protein 12 antibody
      • MCMP antibody
      • MCMPMltna antibody
      • Meltrin alpha antibody
      • Meltrin-alpha antibody
      • MLTN antibody
      • MLTNA antibody
      • OTTHUMP00000046766 antibody
      see all

    Anti-ADAM12 antibody images

    • Western blot against tagged recombinant protein immunogen using ab56366 ADAM12 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    References for Anti-ADAM12 antibody (ab56366)

    ab56366 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - other (Mesenchymal stem cell)
    Gel Running Conditions Reduced Denaturing
    Loading amount 20 µg
    Specification Mesenchymal stem cell
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    Submitted Aug 19 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"