Anti-ADAM12 antibody - Aminoterminal end (ab39155)
Key features and details
- Rabbit polyclonal to ADAM12 - Aminoterminal end
- Suitable for: WB, ICC/IF, IHC-Fr, Flow Cyt
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-ADAM12 antibody - Aminoterminal end
See all ADAM12 primary antibodies -
Description
Rabbit polyclonal to ADAM12 - Aminoterminal end -
Host species
Rabbit -
Specificity
This antibody recognizes latent unprocessed and processed forms of ADAM12, but does not react with other ADAMs. -
Tested applications
Suitable for: WB, ICC/IF, IHC-Fr, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig -
Immunogen
Synthetic peptide corresponding to Human ADAM12 (N terminal).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 2.9% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody has been peptide affinity purified. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 100 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.
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ICC/IF | (3) |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration. PubMed: 23671573
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Flow Cyt |
Use at an assay dependent concentration. PubMed: 19213876
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 100 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. PubMed: 23671573 |
Flow Cyt
Use at an assay dependent concentration. PubMed: 19213876 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. -
Tissue specificity
Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. -
Domain
The cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8038 Human
- Entrez Gene: 11489 Mouse
- Entrez Gene: 100157228 Pig
- Entrez Gene: 679837 Rat
- Omim: 602714 Human
- SwissProt: O43184 Human
- SwissProt: Q61824 Mouse
- Unigene: 594537 Human
see all -
Alternative names
- A disintegrin and metalloproteinase domain 12 antibody
- ADA12_HUMAN antibody
- ADAM 12 antibody
see all
Images
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Anti-ADAM12 antibody - Aminoterminal end (ab39155) at 1/1000 dilution + Human heart tissue lysate - total protein (ab29431) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Predicted band size: 100 kDa
Observed band size: 84 kDa why is the actual band size different from the predicted?
Additional bands at: 117 kDa, 42 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab39155 has been referenced in 8 publications.
- Mallm JP et al. Glioblastoma initiating cells are sensitive to histone demethylase inhibition due to epigenetic deregulation. Int J Cancer 146:1281-1292 (2020). PubMed: 31456217
- Huang X et al. Adam12 and lnc015192 act as ceRNAs in breast cancer by regulating miR-34a. Oncogene N/A:N/A (2018). PubMed: 30042416
- Jiao T et al. Role of MicroRNA-103a Targeting ADAM10 in Abdominal Aortic Aneurysm. Biomed Res Int 2017:9645874 (2017). WB ; Mouse . PubMed: 28357407
- Mendoza-Parra MA et al. Antibody performance in ChIP-sequencing assays: From quality scores of public data sets to quantitative certification. F1000Res 5:54 (2016). PubMed: 27335635
- Brzoska E et al. Sdf-1 (CXCL12) induces CD9 expression in stem cells engaged in muscle regeneration. Stem Cell Res Ther 6:46 (2015). WB, ICC . PubMed: 25890097
- Archacka K et al. Competence of in vitro cultured mouse embryonic stem cells for myogenic differentiation and fusion with myoblasts. Stem Cells Dev 23:2455-68 (2014). PubMed: 24940624
- Przewozniak M et al. Adhesion proteins--an impact on skeletal myoblast differentiation. PLoS One 8:e61760 (2013). WB, IHC-Fr, ICC/IF ; Mouse, Rat . PubMed: 23671573
- Estrella C et al. Role of A disintegrin and metalloprotease-12 in neutrophil recruitment induced by airway epithelium. Am J Respir Cell Mol Biol 41:449-58 (2009). Flow Cyt ; Human . PubMed: 19213876